CRISP3, ELISA Kit

Cysteine-rich secretory protein 3; cysteine-rich secretory protein 3; Aeg 2; Aeg2; CRIS3_HUMAN; CRISP 3; CRISP-3; Crisp3; CRS 3; CRS3; Cysteine rich secretory protein 3; Cysteine-rich secretory protein 3; dJ442L6.3; MGC126588; OTTHUMP00000016590; OTTHUMP00000039911; SGP 28; SGP28; SGP28 protein; Specific granule protein (28 kDa); Specific granule protein of 28 kDa

For Research Use Only. Not for use in diagnostic procedures.

Natural and recombinant human CRISP3

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of CRISP3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS177209 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CRISP3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CRISP3. The ELISA analytical biochemical technique of the MBS177209 kit is based on CRISP3 antibody-CRISP3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CRISP3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CRISP3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: human CRISP3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CRISP3 has been precoated onto 96-well plates. Standards(NSO, N21-Y245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRISP3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CRISP3 amount of sample captured in plate.
Background: Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.

Human CRISP3 PicoKine ELISA Kit standard curve

30,116 Da

cysteine-rich secretory protein 3

cysteine-rich secretory protein 3; cysteine-rich secretory protein-3; specific granule protein (28 kDa); specific granule protein of 28 kDa

Cysteine-rich secretory protein 3

CRISP-3; SGP28  [Similar Products]

CRIS3_HUMAN

CRISP3: Belongs to the CRISP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: extracellular space; proteinaceous extracellular matrix; specific granule; extracellular region

Biological Process: innate immune response; defense response

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