Product Name
SLC34A2, siRNA
Full Product Name
SLC34A2 siRNA (Rat)
Product Synonym Names
Sodium-dependent phosphate transport protein 2B; Sodium-phosphate transport protein 2B; Na(+)-dependent phosphate cotransporter 2B; Sodium/phosphate cotransporter 2B; Na(+)/Pi cotransporter 2B; NaPi-2b; Solute carrier family 34 member 2; rNaPi IIb
Product Gene Name
SLC34A2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9JJ09
Specificity
SLC34A2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SLC34A2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SLC34A2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SLC34A2 sirna
siRNA to inhibit SLC34A2 expression using RNA interference
Applications Tested/Suitable for SLC34A2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SLC34A2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_445832.1
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NCBI GenBank Nucleotide #
NM_053380.2
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UniProt Primary Accession #
Q9JJ09
[Other Products]
UniProt Secondary Accession #
Q8VI55[Other Products]
UniProt Related Accession #
Q9JJ09[Other Products]
Molecular Weight
75,992 Da
NCBI Official Full Name
sodium-dependent phosphate transport protein 2B
NCBI Official Synonym Full Names
solute carrier family 34 (type II sodium/phosphate cotransporter), member 2
NCBI Official Symbol
Slc34a2 [Similar Products]
NCBI Protein Information
sodium-dependent phosphate transport protein 2B
UniProt Protein Name
Sodium-dependent phosphate transport protein 2B
UniProt Synonym Protein Names
Na(+)-dependent phosphate cotransporter 2B; Sodium/phosphate cotransporter 2B; Na(+)/Pi cotransporter 2B; NaPi-2b; Solute carrier family 34 member 2; rNaPi IIb
Protein Family
Sodium-dependent phosphate transport protein
UniProt Gene Name
Slc34a2 [Similar Products]
UniProt Synonym Gene Names
Sodium-phosphate transport protein 2B; Na(+)/Pi cotransporter 2B; NaPi-2b; rNaPi IIb [Similar Products]
UniProt Entry Name
NPT2B_RAT
NCBI Summary for SLC34A2
may act as a sodium dependent phosphate transporter; may provide inorganic phosphate for the synthesis of lung surfactant [RGD, Feb 2006]
UniProt Comments for SLC34A2
SLC34A2: May be involved in actively transporting phosphate into cells via Na(+) cotransport. It may be the main phosphate transport protein in the intestinal brush border membrane. May have a role in the synthesis of surfactant in lungs' alveoli. Defects in SLC34A2 are a cause of pulmonary alveolar microlithiasis (PALM). Pulmonary alveolar microlithiasis is a rare disease characterized by the deposition of calcium phosphate microliths throughout the lungs. Most patients are asymptomatic for several years or even for decades and generally, the diagnosis is incidental to clinical investigations unrelated to the disease. Cases with early onset or rapid progression are rare. A 'sandstorm-appearing' chest roentgenogram is a typical diagnostic finding. The onset of this potentially lethal disease varies from the neonatal period to old age and the disease follows a long-term, progressive course, resulting in a slow deterioration of lung functions. Pulmonary alveolar microlithiasis is a recessive monogenic disease with full penetrance. A chromosomal aberration involving SLC34A2 is found in a non-small cell lung cancer cell line. Results in the formation of a SLC34A2-ROS1 chimeric protein that retains a constitutive kinase activity. Belongs to the SLC34A transporter family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter, SLC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral
Cellular Component: microvillus membrane; brush border membrane; apical plasma membrane; integral to membrane; brush border; vesicle
Molecular Function: protein domain specific binding; sodium ion binding; phosphate binding; sodium-dependent phosphate transmembrane transporter activity; sodium:phosphate symporter activity
Biological Process: cellular phosphate ion homeostasis; phosphate transport; response to estrogen stimulus; in utero embryonic development; response to fructose stimulus; response to estradiol stimulus; aging
Research Articles on SLC34A2
1. that NHERF1 associates with NaPi-2b in enterocytes and regulates NaPi-2b adaptation.
Precautions
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Disclaimer
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