Calcitonin Receptor, Monoclonal Antibody

Anti -Calcitonin Receptor (CRT, CTR, CT-R, CTR1, CALCR)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 7; NC_000007.13 (93053799..93204042, complement). Location: 7q21.3

Monoclonal

IgG2a

9j1

Mouse

Recognizes human calcitonin receptor. Stains human CALCR transfectants but not irrelevant transfectants.

Affinity Purified
Purified by Protein G affinity chromatography.

Supplied as a sterile-filtered lyophilized powder in PBS, pH 7.2, 5% trehalose. Reconstitution: sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 0.5mg/ml.

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-CALCR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Calcitonin receptor (CALCR) is a glycosylated 70 kDa seven-transmembrane G protein-coupled receptor that mediates the hypocalcemic effects of the peptide hormone, calcitonin. CALCR activation inhibits osteoclast-mediated bone resorption and enhances renal calcium excretion. CALCR polymorphisms and mutations have been associated with several bone disorders. Alternate splicing results in the deletion of 16 aa in the first cytoplasmic loop or 23 aa in the first extracellular region. Human CALCR shares 70%-72% aa sequence identity with mouse and rat CACLR.

Antibodies; Abs to Receptors

Flow Cytometry (FC/FACS)

Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 0.25ug/ml. Validated for flow cytometry using MCF-7 cells. Dilute this antibody to 25ug/ml and add 10ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome.
MCF-7 cells were stained with anti-CALCR (filled histogram) or isotype control (open histogram) followed by PE-conjugated anti-mouse antibody.

57,303 Da[Similar Products]

calcitonin receptor

calcitonin receptor; OTTHUMP00000162535; OTTHUMP00000208683; OTTHUMP00000208686

Calcitonin receptor

CALCR_HUMAN

This gene encodes a high affinity receptor for the peptide hormone calcitonin and belongs to a subfamily of seven transmembrane-spanning G protein-coupled receptors. The encoded protein is involved in maintaining calcium homeostasis and in regulating osteoclast-mediated bone resorption. Polymorphisms in this gene have been associated with variations in bone mineral density and onset of osteoporosis. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

CALCR: This is a receptor for calcitonin. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The calcitonin receptor is thought to couple to the heterotrimeric guanosine triphosphate-binding protein that is sensitive to cholera toxin. Belongs to the G-protein coupled receptor 2 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 2; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; calcitonin binding; receptor activity; protein transporter activity; calcitonin receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; protein transport; phospholipase C activation; receptor internalization; positive regulation of cAMP biosynthetic process; response to glucocorticoid stimulus; adenylate cyclase activation; positive regulation of adenylate cyclase activity; G-protein signaling, adenylate cyclase activating pathway

Disease: Osteoporosis

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.