Glycogen Phosphorylase, Muscle, ELISA Kit

PYGM

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of PYGM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS760405 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glycogen Phosphorylase, Muscle (PYGM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PYGM. The ELISA analytical biochemical technique of the MBS760405 kit is based on PYGM antibody-PYGM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PYGM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PYGM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat PYGM antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat PYGM antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat PYGM amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat PYGM can be calculated.

87,317 Da

phosphorylase, glycogen, muscle

glycogen phosphorylase, muscle form

Glycogen phosphorylase, muscle form

PYGM_HUMAN

This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]

PYGM: an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Phosphorylation of Ser-14 converts phosphorylase B (unphosphorylated) to phosphorylase A.

Protein type: Carbohydrate Metabolism - starch and sucrose; Phosphorylase; EC 2.4.1.1; Transferase; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 11q12-q13.2

Cellular Component: cytoplasm; cytosol; sarcoplasmic reticulum

Molecular Function: AMP binding; carbohydrate binding; drug binding; glycogen phosphorylase activity; protein binding; pyridoxal phosphate binding

Biological Process: carbohydrate metabolic process; cellular calcium ion homeostasis; glucose metabolic process; glycogen catabolic process; glycogen metabolic process; response to cAMP; response to hypoxia

Disease: Glycogen Storage Disease V

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