Product Name
Cardiac Troponin I (TNNI3), Monoclonal Antibody
Full Product Name
Cardiac Troponin I Monoclonal Antibody
Product Synonym Names
Anti-Cardiac Troponin I; Cardiac Troponin I
Product Gene Name
anti-TNNI3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 19; NC_000019.9 (55663135..55669100, complement). Location: 19q13.4
3D Structure
ModBase 3D Structure for P19429
Specificity
Anti-Cardiac Troponin I [Murine MAbs recognize human cardiac troponin I and do not cross-react with human skeletal troponin (2 clones).]
Form/Format
100 ug Protein A-purified antibody in PBS, pH 7.4.
Antigen
Purified human cardiac troponin I.
Activity and Specificity
These antibodies react specifically with human cardiac troponin I. There is no cross-reactivity with human skeletal troponin I. ELISA titers > 1: 1,000,000.
Preservatives
0.1% sodium azide.
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
These antibodies are stable for at least one (1) year at -20 degree C to -70 degree C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles
Other Notes
Small volumes of anti-TNNI3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-TNNI3 antibody
Hormone and Serum Protein Antibodies
Applications Tested/Suitable for anti-TNNI3 antibody
Western Blot, Immunoassay
Application Notes for anti-TNNI3 antibody
These antibodies may be used in immunoassays and Western Blots to detect and quantitate human cardiac troponin I. They may also be used to affinity purify cardiac troponin I.
NCBI/Uniprot data below describe general gene information for TNNI3. It may not necessarily be applicable to this product.
NCBI Accession #
CAA62301.1
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UniProt Primary Accession #
P19429
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UniProt Related Accession #
P19429; Q6FGU5; Q6FGX2[Other Products]
Molecular Weight
24,008 Da[Similar Products]
NCBI Official Full Name
cardiac troponin I
NCBI Official Synonym Full Names
troponin I type 3 (cardiac)
NCBI Official Symbol
TNNI3 [Similar Products]
NCBI Official Synonym Symbols
CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; MGC116817
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NCBI Protein Information
troponin I, cardiac muscle
UniProt Protein Name
Troponin I, cardiac muscle
UniProt Synonym Protein Names
Cardiac troponin I
UniProt Gene Name
TNNI3 [Similar Products]
UniProt Synonym Gene Names
TNNC1 [Similar Products]
UniProt Entry Name
TNNI3_HUMAN
NCBI Summary for TNNI3
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]
UniProt Comments for TNNI3
TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.
Protein type: Motor; Actin-binding; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 19q13.4
Cellular Component: sarcomere; troponin complex; cytosol
Molecular Function: troponin T binding; protein domain specific binding; troponin C binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; calcium-dependent protein binding; protein kinase binding
Biological Process: cellular calcium ion homeostasis; regulation of smooth muscle contraction; heart contraction; heart development; regulation of systemic arterial blood pressure by ischemic conditions; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; cardiac muscle contraction; muscle filament sliding
Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a
Research Articles on TNNI3
1. Immunoblotting of blood samples from patients with acute ischemic stroke reveals similar degradation patterns of cTnI as has been described in patients with acute myocardial infarction.
Precautions
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Disclaimer
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