Full Product Name
SIL1 siRNA (Human)
Product Synonym Names
Nucleotide exchange factor SIL1; BiP-associated protein; BAP
Product Gene Name
SIL1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9H173
Specificity
SIL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SIL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SIL1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SIL1 sirna
siRNA to inhibit SIL1 expression using RNA interference
Applications Tested/Suitable for SIL1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SIL1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001032722.1
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NCBI GenBank Nucleotide #
NM_001037633.1
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UniProt Primary Accession #
Q9H173
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UniProt Secondary Accession #
Q8N2L3; D3DQC2[Other Products]
UniProt Related Accession #
Q9H173[Other Products]
Molecular Weight
52,085 Da
NCBI Official Full Name
nucleotide exchange factor SIL1
NCBI Official Synonym Full Names
SIL1 nucleotide exchange factor
NCBI Official Symbol
SIL1 [Similar Products]
NCBI Official Synonym Symbols
BAP; MSS; ULG5
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NCBI Protein Information
nucleotide exchange factor SIL1
UniProt Protein Name
Nucleotide exchange factor SIL1
UniProt Synonym Protein Names
BiP-associated protein; BAP
UniProt Gene Name
SIL1 [Similar Products]
UniProt Synonym Gene Names
BAP [Similar Products]
UniProt Entry Name
SIL1_HUMAN
NCBI Summary for SIL1
This gene encodes a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
UniProt Comments for SIL1
SIL1: Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5. Defects in SIL1 are a cause of Marinesco-Sjoegren syndrome (MSS). MSS is an autosomal recessive multisystem disorder which is characterized by cerebellar ataxia due to cerebellar atrophy, with Purkinje and granule cell loss and myopathy featuring marked muscle replacement with fat and connective tissue. Other cardinal features include bilateral cataracts, hypergonadotrophic hypogonadism and mild to severe mental retardation. Skeletal abnormalities, short stature, dysarthria, strabismus and nystagmus are also frequent findings. Mutational inactivation of this protein may result in ER stress- induced cell death signaling or malfunctioning chaperone machineries that mishandle client proteins which are critical for the organs targeted in MSS. Belongs to the SIL1 family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: extracellular space; endoplasmic reticulum lumen; endoplasmic reticulum
Molecular Function: unfolded protein binding
Biological Process: intracellular protein transport; protein folding
Disease: Marinesco-sjogren Syndrome
Research Articles on SIL1
1. This study demonistrated that SIL1 mutation in patient with ataxia telangiectasia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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