GDF5, Recombinant Protein

Growth/differentiation factor 5 precursor; Growth/differentiation factor 5 precursor; bp; brp; Cdmp-1

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 90% by SDS-PAGE

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol

0.5 mg/ml (determined by Bradford assay) (lot specific)

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of GDF5 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Growth/differentiation factor 5 precursor, also known as GDF5, is a member of the TGF superfamily. GDF5 has been shown to induce activation of plasminogen activator, thereby inducing angiogenesis. It is predominantly expressed in long bones during fetal embryonic development and is involved in bone formation. GDF5mutations have been identified in mice with the mutation brachypodism (bp), a mutation which affects the length and number of bones in limbs. Recombinant mouse GDF5 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Hormones & Cytokines

SDS-PAGE

16kDa (143aa)

growth differentiation factor 5

growth/differentiation factor 5

Growth/differentiation factor 5

Bmp14; Bp; Gdf-5; GDF-5; BMP-14  [Similar Products]

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates the development of numerous tissue and cell types, including cartilage, joints, brown fat, teeth, and the growth of neuronal axons and dendrites. Mice with a mutation in this gene exhibit enhanced tooth enamel formation. [provided by RefSeq, Aug 2016]

Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction (). Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (). Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (PubMed:24076600). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes ().

Katayama R., et al. (2004) Rheumatology. 43: 980-985.; Kwong F N., et al. (2008) J Orthop Res. 27: 752-757.;

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