Estrogen Receptor beta (ERbeta), ELISA Kit

Estrogen Receptor-beta (Human) ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C for 6 months.

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Small volumes of ERbeta elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS846744 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Estrogen Receptor beta (ERbeta) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ERbeta. The ELISA analytical biochemical technique of the MBS846744 kit is based on ERbeta antibody-ERbeta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ERbeta antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ERbeta. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Background/Introduction: Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ER-beta ELISA kit is a sandwich ELISA assay for the quantitative measurement of human ER-beta in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of human ER-betacaptured from the samples.

47,904 Da

estrogen receptor 2

estrogen receptor beta

Estrogen receptor beta

Erbeta; Nr3a2; ER-beta  [Similar Products]

binds estrogen and mediates transcriptional activation [RGD, Feb 2006]

ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 6q24

Cellular Component: cell soma; cilium; cytoplasm; mitochondrion; neuron projection; nucleus; perikaryon; perinuclear region of cytoplasm; plasma membrane

Molecular Function: DNA binding; drug binding; enzyme binding; estrogen receptor activity; estrogen response element binding; hormone binding; peroxisome proliferator activated receptor binding; protein binding; sequence-specific DNA binding; steroid binding; steroid hormone receptor activity

Biological Process: amygdala development; behavioral fear response; brain development; cerebellum development; estrogen receptor signaling pathway; female gonad development; hypothalamus development; induction of apoptosis by hormones; learning and/or memory; male gonad development; negative regulation of behavior; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; neuron migration; ovarian follicle development; positive regulation of apoptosis; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; prostate gland development; regulation of cell proliferation; regulation of neuron apoptosis; response to activity; response to drug; response to estradiol stimulus; response to estrogen stimulus; response to ethanol; response to genistein; response to hormone stimulus; response to insecticide; response to nutrient levels; response to organic cyclic substance; response to testosterone stimulus; response to water deprivation; Sertoli cell development; Sertoli cell proliferation; steroid hormone receptor signaling pathway; uterus development; vagina development

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