Full Product Name
GDF5 siRNA (Human)
Product Synonym Names
BMP14; CDMP1; Growth/differentiation factor 5; GDF-5; Bone morphogenetic protein 14; BMP-14; Cartilage-derived morphogenetic protein 1; CDMP-1; Lipopolysaccharide-associated protein 4; LAP-4; LPS-associated protein 4; Radotermin
Product Gene Name
GDF5 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P43026
Specificity
GDF5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GDF5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GDF5 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GDF5 sirna
siRNA to inhibit GDF5 expression using RNA interference
Applications Tested/Suitable for GDF5 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GDF5. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000548.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000557.4
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UniProt Primary Accession #
P43026
[Other Products]
UniProt Secondary Accession #
Q96SB1; E1P5Q2[Other Products]
UniProt Related Accession #
P43026[Other Products]
Molecular Weight
55,411 Da
NCBI Official Full Name
growth/differentiation factor 5 preproprotein
NCBI Official Synonym Full Names
growth differentiation factor 5
NCBI Official Symbol
GDF5 [Similar Products]
NCBI Official Synonym Symbols
OS5; LAP4; BDA1C; BMP14; CDMP1; LAP-4; SYM1B; SYNS2; BMP-14
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NCBI Protein Information
growth/differentiation factor 5
UniProt Protein Name
Growth/differentiation factor 5
UniProt Synonym Protein Names
Bone morphogenetic protein 14; BMP-14; Cartilage-derived morphogenetic protein 1; CDMP-1; Lipopolysaccharide-associated protein 4; LAP-4; LPS-associated protein 4; Radotermin
Protein Family
Growth/differentiation factor
UniProt Gene Name
GDF5 [Similar Products]
UniProt Synonym Gene Names
BMP14; CDMP1; GDF-5; BMP-14; CDMP-1; LAP-4; LPS-associated protein 4 [Similar Products]
UniProt Entry Name
GDF5_HUMAN
NCBI Summary for GDF5
The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and ***** tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development. [provided by RefSeq, Jul 2008]
UniProt Comments for GDF5
GDF5: a cytokine that is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. These cytokines are characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and ***** tissues. Binds to bone morphogenetic protein receptors (BMPRs), a family of transmembrane serine/threonine kinases. BMPRs are involved in bone and cartilage formation. Chondrogenic signaling is mediated by the high-affinity receptor BMPR1B. Defects in GDF5 are the cause of acromesomelic chondrodysplasia Grebe type (AMDG), acromesomelic chondrodysplasia Hunter-Thompson type (AMDH), brachydactyly type C (BDC), Du Pan syndrome (DPS), symphalangism proximal syndrome (SYM1), multiple synostoses syndrome type 2 (SYNS2), and brachydactyly type A2 (BDA2). Genetic variations in GDF5 are associated with susceptibility to osteoarthritis type 5 (OS5). Belongs to the TGF-beta family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 20q11.2
Cellular Component: extracellular space; plasma membrane; extracellular region
Molecular Function: identical protein binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding
Biological Process: hindlimb morphogenesis; extracellular matrix organization and biogenesis; regulation of multicellular organism growth; regulation of apoptosis; BMP signaling pathway; forelimb morphogenesis; positive regulation of chondrocyte differentiation; cell-cell signaling; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; chondrocyte differentiation; negative regulation of neuron apoptosis; positive regulation of neuron differentiation; cell development; negative regulation of epithelial cell proliferation; embryonic limb morphogenesis; growth
Disease: Chondrodysplasia, Grebe Type; Acromesomelic Dysplasia, Hunter-thompson Type; Brachydactyly, Type A1, C; Brachydactyly, Type A2; Symphalangism, Proximal, 1b; Brachydactyly, Type C; Fibular Hypoplasia And Complex Brachydactyly; Osteoarthritis Susceptibility 5; Multiple Synostoses Syndrome 2
Research Articles on GDF5
1. This meta-analysis finds that the C allele and CC genotype of the GDF5 gene are protective for knee osteoarthritis susceptibility.
Precautions
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Disclaimer
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