Full Product Name
CLNK siRNA (Human)
Product Synonym Names
MIST; Cytokine-dependent hematopoietic cell linker; Mast cell immunoreceptor signal transducer
Product Gene Name
CLNK sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q7Z7G1
Specificity
CLNK siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CLNK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CLNK sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CLNK sirna
siRNA to inhibit CLNK expression using RNA interference
Applications Tested/Suitable for CLNK sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CLNK. It may not necessarily be applicable to this product.
NCBI Accession #
NP_443196.2
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NCBI GenBank Nucleotide #
NM_052964.2
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UniProt Primary Accession #
Q7Z7G1
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UniProt Secondary Accession #
Q05C27; Q9P2U9[Other Products]
UniProt Related Accession #
Q7Z7G1[Other Products]
Molecular Weight
45,469 Da
NCBI Official Full Name
cytokine-dependent hematopoietic cell linker
NCBI Official Synonym Full Names
cytokine-dependent hematopoietic cell linker
NCBI Official Symbol
CLNK [Similar Products]
NCBI Official Synonym Symbols
MIST
[Similar Products]
NCBI Protein Information
cytokine-dependent hematopoietic cell linker
UniProt Protein Name
Cytokine-dependent hematopoietic cell linker
UniProt Synonym Protein Names
Mast cell immunoreceptor signal transducer
Protein Family
Cytokine-dependent hematopoietic cell linker
UniProt Gene Name
CLNK [Similar Products]
UniProt Synonym Gene Names
MIST [Similar Products]
UniProt Entry Name
CLNK_HUMAN
NCBI Summary for CLNK
MIST is a member of the SLP76 family of adaptors (see LCP2, MIM 601603; BLNK, MIM 604515). MIST plays a role in the regulation of immunoreceptor signaling, including PLC-gamma (PLCG1; MIM 172420)-mediated B cell antigen receptor (BCR) signaling and FC-epsilon R1 (see FCER1A, MIM 147140)-mediated mast cell degranulation (Cao et al., 1999 [PubMed 10562326]; Goitsuka et al., 2000, 2001 [PubMed 10744659] [PubMed 11463797]).[supplied by OMIM, Mar 2008]
UniProt Comments for CLNK
MIST: Plays a role in the regulation of immunoreceptor signaling, including PLC-gamma-mediated B-cell antigen receptor (BCR) signaling and FC-epsilon R1-mediated mast cell degranulation. Involved in phosphorylation of LAT. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 4p16.1
Cellular Component: intracellular
Molecular Function: protein binding; SH3/SH2 adaptor activity
Biological Process: positive regulation of signal transduction; immune response; transmembrane receptor protein tyrosine kinase signaling pathway
Research Articles on CLNK
1. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
