Product Name
Parathyroid Hormone (PTH), Recombinant Protein
Popular Item
Full Product Name
Human Parathyroid Hormone Recombinant
Product Synonym Names
pth; Parathyroid
Product Gene Name
PTH recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MIPAKDMAKV MIVMLAICFL TKSDGKSVKK RSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ
3D Structure
ModBase 3D Structure for P01270
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant PTH-84 was lyophilized from a 0.2 um filtered PB solution.
Host Note
Optimized DNA sequence encoding Human PTH mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Human PTH, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 9 kDa. Recombinant Human PTH-84 is a disulfide-linked homodimeric protein consisting of 84 amino acid residue subunits, and migrates as an approximately 9 kDa under reducing conditions in SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of PTH recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PTH recombinant protein
Parathyroid hormone is a circulating hormone that acts as the central regulator of calcium metabolism by directly targeting bone, kidney, and intestine. The classical concept of PTH action is that it regulates serum calcium levels by stimulating bone resorption; however, intermittent administration of PTH selectively stimulates bone formation. It is now known that PTH binds to its receptor PTH1R, and activates the G protein α subunits.Besides PKA and PKC activation, PTH also regulates MAPKs including p42/p44 ERKs, p38, and c-Jun N-terminal kinase subtypes.
Product Categories/Family for PTH recombinant protein
Cytokines
NCBI/Uniprot data below describe general gene information for PTH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000306.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000315.2
[Other Products]
UniProt Primary Accession #
P01270
[Other Products]
UniProt Secondary Accession #
Q4VB48; Q9UD38[Other Products]
UniProt Related Accession #
P01270[Other Products]
Molecular Weight
12,861 Da
NCBI Official Full Name
parathyroid hormone preproprotein
UniProt Protein Name
Parathyroid hormone
UniProt Synonym Protein Names
Parathormone; Parathyrin
Protein Family
Parathyroid hormone
UniProt Gene Name
PTH [Similar Products]
UniProt Synonym Gene Names
PTH [Similar Products]
UniProt Entry Name
PTHY_HUMAN
NCBI Summary for PTH
The protein encoded by this gene is a hormone secreted by parathyroid cells. This hormone elevates blood Ca2+ level by dissolving the salts in bone and preventing their renal excretion. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH). [provided by RefSeq, Jul 2008]
UniProt Comments for PTH
PTH: PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2- deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells. Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH); also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism. Belongs to the parathyroid hormone family.
Protein type: Secreted; Secreted, signal peptide; Apoptosis; Hormone
Chromosomal Location of Human Ortholog: 11p15.3-p15.1
Cellular Component: extracellular space; extracellular region
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; type 1 parathyroid hormone receptor binding; peptide hormone receptor binding; parathyroid hormone receptor binding; hormone activity
Biological Process: response to drug; transcription from RNA polymerase II promoter; positive regulation of signal transduction; induction of apoptosis by hormones; positive regulation of glycogen biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; positive regulation of bone mineralization; G-protein signaling, adenylate cyclase activating pathway; Rho protein signal transduction; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of glucose import; response to ethanol; response to vitamin D; response to cadmium ion; cAMP metabolic process; cell-cell signaling; regulation of gene expression; response to lead ion; positive regulation of cAMP biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; skeletal development; bone resorption
Disease: Hypoparathyroidism, Familial Isolated
Product References and Citations for PTH recombinant protein
• endogenous parathyroid hormone-related protein regulates the expression of PTH type 1 receptor and proliferation of vascular smooth muscle cells .Mol. Endocrinol., Oct 2009; 23: 1681 - 1690.
• PTH-induced internalization of apical membrane napi2a: role of actin and myosin vi .Am J Physiol Cell Physiol, Sep 2009; 10.1152/ajpcell.00260.2009.
• motoring down the microvilli. editorial focus on "PTH-induced internalization of apical membrane napi2a: role of actin and myosin vi" .Am J Physiol Cell Physiol, Sep 2009; 10.1152/ajpcell.00423.2009.
• simultaneous control of PTH and caxp is sustained over three years of treatment with cinacalcet hcl .Clin. J. Am. Soc. Nephrol., Sep 2009; 4: 1465 - 1476.
• human PTH gene regulation in vivo using transgenic mice .Am J Physiol Renal Physiol, Sep 2009; 297: F713 - F719.
Precautions
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