Product Name
CACNA1C, siRNA
Full Product Name
CACNA1C siRNA (Rat)
Product Synonym Names
CACH2; CACN2; CACNL1A1; CCHL1A1; Voltage-dependent L-type calcium channel subunit alpha-1C; Calcium channel. L type. alpha-1 polypeptide isoform 1. cardiac muscle; Rat brain class C; RBC; Voltage-gated calcium channel subunit alpha Cav1.2
Product Gene Name
CACNA1C sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P22002
Specificity
CACNA1C siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CACNA1C gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CACNA1C sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CACNA1C sirna
siRNA to inhibit CACNA1C expression using RNA interference
Applications Tested/Suitable for CACNA1C sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CACNA1C. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036649.2
[Other Products]
NCBI GenBank Nucleotide #
NM_012517.2
[Other Products]
UniProt Primary Accession #
P22002
[Other Products]
UniProt Secondary Accession #
P27733; P27734; Q62816; Q63271; Q64178[Other Products]
UniProt Related Accession #
P22002[Other Products]
Molecular Weight
240,488 Da
NCBI Official Full Name
voltage-dependent L-type calcium channel subunit alpha-1C
NCBI Official Synonym Full Names
calcium channel, voltage-dependent, L type, alpha 1C subunit
NCBI Official Symbol
Cacna1c [Similar Products]
NCBI Official Synonym Symbols
RATIVS302
[Similar Products]
NCBI Protein Information
voltage-dependent L-type calcium channel subunit alpha-1C
UniProt Protein Name
Voltage-dependent L-type calcium channel subunit alpha-1C
UniProt Synonym Protein Names
Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle; Rat brain class C; RBC; Voltage-gated calcium channel subunit alpha Cav1.2
Protein Family
Voltage-dependent L-type calcium channel
UniProt Gene Name
Cacna1c [Similar Products]
UniProt Synonym Gene Names
Cach2; Cacn2; Cacnl1a1; Cchl1a1; RBC [Similar Products]
UniProt Entry Name
CAC1C_RAT
NCBI Summary for CACNA1C
subunit of an L-type calcium channel that may be involved in bone metabolism and compensatory renal growth [RGD, Feb 2006]
UniProt Comments for CACNA1C
CACNA1C: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin- GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function. Defects in CACNA1C are the cause of Timothy syndrome (TS). TS is a disorder characterized by multiorgan dysfunction including lethal arrhythmias, webbing of fingers and toes, congenital heart disease, immune deficiency, intermittent hypoglycemia, cognitive abnormalities and autism. Defects in CACNA1C are the cause of Brugada syndrome type 3 (BRGDA3). A heart disease characterized by the association of Brugada syndrome with shortened QT intervals. Brugada syndrome is a tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily. 35 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Channel, calcium; Membrane protein, integral
Cellular Component: protein complex; cell; T-tubule; postsynaptic density; dendrite; integral to membrane; caveolar macromolecular signaling complex; Z disc; presynaptic membrane; cell soma; membrane; cytoplasm; plasma membrane; voltage-gated calcium channel complex; dendritic shaft; nucleus; sarcolemma
Molecular Function: voltage-gated calcium channel activity; calmodulin binding; protein domain specific binding; protein binding; enzyme binding; protein phosphatase 2A binding; metal ion binding; high voltage-gated calcium channel activity; alpha-actinin binding
Biological Process: regulation of organ growth; glucose homeostasis; ***** walking behavior; calcium ion-dependent exocytosis; cellular calcium ion homeostasis; synaptic transmission; growth hormone secretion; elevation of cytosolic calcium ion concentration; smooth muscle contraction; smooth muscle contraction involved in micturition; insulin secretion; regulation of blood pressure; calcium ion transport; regulation of vasoconstriction; visual learning
Research Articles on CACNA1C
1. LTCCalpha 1C is a vascular smooth muscle cell contractile phenotype marker gene.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
