Pituitary-specific positive transcription factor 1 (POU1F1), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of POU1F1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7230736 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pituitary-specific positive transcription factor 1 (POU1F1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing POU1F1. The ELISA analytical biochemical technique of the MBS7230736 kit is based on POU1F1 antibody-POU1F1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect POU1F1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, POU1F1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Epigenetics and Nuclear Signaling

35,669 Da

POU class 1 homeobox 1

pituitary-specific positive transcription factor 1; growth hormone factor 1; POU domain, class 1, transcription factor 1

Pituitary-specific positive transcription factor 1

GHF1; PIT1; PIT-1; GHF-1  [Similar Products]

PIT1_HUMAN

This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

GHF-1: Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'- TAAAT-3'. Belongs to the POU transcription factor family. Class- 1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 3p11

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: chromatin binding; transcription factor activity; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; nuclear transport; regulation of insulin-like growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; cell fate specification; positive regulation of inositol trisphosphate biosynthetic process; positive regulation of multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; determination of ***** life span; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; B cell differentiation; somatotropin secreting cell development; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter

Disease: Pituitary Hormone Deficiency, Combined, 1

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