MyoD, Polyclonal Antibody

Anti -MyoD (Myogenic Determination)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 11; NC_000011.9 (17741110..17743678). Location: 11p15.4

Polyclonal

IgG

Rabbit

Recognizes human MyoD. Species Crossreactivity: mouse, rat.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-MYOD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

Antibodies; Abs to Cancer Markers

Western Blot (WB)

Suitable for use in Western Blot.
Dilution: Western Blot: 1:500-1:1,000

34,501 Da[Similar Products]

myogenic differentiation 1

myoblast determination protein 1; myf-3; myogenic factor 3; OTTHUMP00000232717; class C basic helix-loop-helix protein 1

Myoblast determination protein 1

BHLHC1; MYF3; MYOD  [Similar Products]

MYOD1_HUMAN

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq]

MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.

Protein type: Transcription factor; RNA splicing; DNA-binding

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; transcription factor complex; myofibril; nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein heterodimerization activity; chromatin DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; nuclear hormone receptor binding

Biological Process: transcription from RNA polymerase II promoter; muscle development; skeletal muscle development; skeletal muscle fiber adaptation; positive regulation of skeletal muscle regeneration; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; muscle cell fate commitment; myotube cell development; skeletal muscle fiber development; cellular response to starvation; protein amino acid phosphorylation; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; myoblast cell fate determination; regulation of alternative nuclear mRNA splicing, via spliceosome

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