Necdin, Polyclonal Antibody

Anti -Necdin

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 15; NC_000015.9 (23930554..23932450, complement). Location: 15q11.2-q12

Polyclonal

IgG

Rabbit

Recognizes human Necdin at ~38kD. Non-specific proteins were also detected, Mr 70 and 100kD, detection of which were inhibited by preincubation with the immunizing peptide. Species Crossreactivity: mouse and rat

Affinity Purified
Purified by Protein A affinity chromatography.

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before glycerol was added to 30%.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-NDN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Necdin is known to be a growth suppressor protein that is a member of the melanoma-associated antigen (MAGE) family and is mainly expressed in postmitotic neurons. Any expression of this protein ectopically halts growth. Necdin has been shown to play a role in modulating gene transcription. Nectin suppresses cell proliferation by interacting with hnRNP U. Necdin seems to be an important protein in the pathogenesis of Prader-Willi syndrome. Recently, Necdin has been linked to fetal and postnatal physiological myogenesis and regeneration of muscle.

Antibodies; Abs to Neuroscience

Western Blot (WB)

Suitable for use in Western Blot.
Dilution: Western Blot: 1ug/ml detects Necdin (~38kD) in NIH3T3 cells. NIH3T3 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with N0571. Proteins were visualized using a goat-anti rabbit IgG (HRP) and a chemiluminescence detection system.

36,086 Da[Similar Products]

necdin homolog (mouse)

necdin; OTTHUMP00000159437

Necdin

NECD_HUMAN

This intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mouse suggest that the protein encoded by this gene may suppress growth in postmitotic neurons. [provided by RefSeq]

NDN: Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle- promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 15q11.2-q12

Cellular Component: centrosome; cell projection; perikaryon; nucleus; cytosol

Molecular Function: gamma-tubulin binding; DNA binding

Biological Process: nervous system development; central nervous system development; nerve growth factor receptor signaling pathway; transcription, DNA-dependent; axon extension; neuron migration; axonal fasciculation; multicellular organismal homeostasis; sensory perception of pain; glial cell migration; post-embryonic development; negative regulation of cell proliferation; respiratory system process; regulation of transcription, DNA-dependent; regulation of growth

Disease: Prader-willi Syndrome

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.