Product Name
SNRPN, siRNA
Full Product Name
SNRPN siRNA (Mouse)
Product Synonym Names
SMN; Small nuclear ribonucleoprotein-associated protein N; snRNP-N; Sm protein D; Sm-D; Sm protein N; Sm-N; SmN; Tissue-specific-splicing protein
Product Gene Name
SNRPN sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P63163
Specificity
SNRPN siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SNRPN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SNRPN sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SNRPN sirna
siRNA to inhibit SNRPN expression using RNA interference
Applications Tested/Suitable for SNRPN sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SNRPN. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001076430.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001082961.1
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UniProt Primary Accession #
P63163
[Other Products]
UniProt Secondary Accession #
P14648; P17135[Other Products]
UniProt Related Accession #
P63163[Other Products]
Molecular Weight
24,614 Da
NCBI Official Full Name
small nuclear ribonucleoprotein-associated protein N
NCBI Official Synonym Full Names
small nuclear ribonucleoprotein N
NCBI Official Symbol
Snrpn [Similar Products]
NCBI Official Synonym Symbols
SMN; Peg4; Pwcr1; HCERN3; 2410045I01Rik
[Similar Products]
NCBI Protein Information
small nuclear ribonucleoprotein-associated protein N
UniProt Protein Name
Small nuclear ribonucleoprotein-associated protein N
UniProt Synonym Protein Names
Sm protein D; Sm-D; Sm protein N; Sm-N; SmN; Tissue-specific-splicing protein
Protein Family
SNRPN upstream reading frame protein
UniProt Gene Name
Snrpn [Similar Products]
UniProt Synonym Gene Names
Smn; snRNP-N; Sm-D; Sm-N; SmN [Similar Products]
UniProt Entry Name
RSMN_MOUSE
NCBI Summary for SNRPN
The protein encoded by this gene is one polypeptide of a small nuclear ribonucleoprotein complex, and it plays a role in pre-mRNA processing. Although individual snRNPs are believed to recognize specific nucleic acid sequences through RNA-RNA base pairing, the specific role of this family member is unknown. This protein arises from a bicistronic transcript that also encodes a protein identified as the Snrpn upstream reading frame (Snurf). Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5' untranslated region. Additional splice variants have been described but sequences for the complete transcripts have not been determined. The 5' UTR of this gene has been identified as an imprinting center. Alternative splicing or deletion caused by a translocation event in this paternally-expressed region in human and mouse is responsible for Angelman syndrome or Prader-Willi syndrome due to parental imprint switch failure. [provided by RefSeq, Jul 2008]
UniProt Comments for SNRPN
snRNP N: May be involved in tissue-specific alternative RNA processing events. Belongs to the snRNP SmB/SmN family.
Protein type: RNA-binding
Cellular Component: snRNP U2; snRNP U1; nucleus
Research Articles on SNRPN
1. Oocytes vitrification could lead to the loss of DNA methylation of imprinted genes (H19, Peg3, and Snrpn) in mouse blastocysts, which is mainly caused by the reductions of DNMTs after vitrification of oocytes.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
