Lamin A/C, Polyclonal Antibody

LMNA; LMN1; Prelamin-A/C

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Antigen Affinity Purified

1 mg/ml (lot specific)

Store at -20 degree C. Avoid freeze / thaw cycles.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Small volumes of anti-LMNA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Cell biology; Epigenetics

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)

WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-1:100, ELISA: 1:5000-1:20000

Western Blot analysis of various cells using Lamin A/C Polyclonal Antibody at dilution of 1:2000.

Immunohistochemistry of paraffin-embedded Human uterus tissue using Lamin A/C Polyclonal Antibody at dilution of 1:200.

Immunofluorescence analysis of Human liver tissue using Lamin A/C Polyclonal Antibody at dilution of 1:200.

Calculated MW: 74
Observed MW: 74+65[Observed+MW:+74+65" title="Find similar products with Calculated MW: 74
Observed MW: 74+65">Similar Products]

lamin A/C

lamin

Prelamin-A/C

LMN1  [Similar Products]

LMNA_HUMAN

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]

lamin A/C: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. Cleaved by caspase-6 during apoptosis into a 40-45 kDa and a28 kDa fragment.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: cytoplasm; cytosol; intermediate filament; lamin filament; nuclear envelope; nuclear lamina; nuclear matrix; nuclear membrane; nuclear speck; nucleoplasm; nucleus; perinuclear region of cytoplasm

Molecular Function: protein binding; protein phosphatase 1 binding; structural molecule activity

Biological Process: apoptosis; cell structure disassembly during apoptosis; cellular protein metabolic process; establishment and/or maintenance of microtubule cytoskeleton polarity; mitotic cell cycle; mitotic nuclear envelope disassembly; mitotic nuclear envelope reassembly; muscle development; positive regulation of osteoblast differentiation; programmed cell death; regulation of cell migration; response to mechanical stimulus; spermatogenesis; sterol regulatory element binding protein nuclear translocation; unfolded protein response; ventricular cardiac muscle cell development

Disease: Cardiomyopathy, Dilated, 1a; Cardiomyopathy, Dilated, With Hypergonadotropic Hypogonadism; Charcot-marie-tooth Disease, Axonal, Type 2b1; Emery-dreifuss Muscular Dystrophy 2, Autosomal Dominant; Emery-dreifuss Muscular Dystrophy 3, Autosomal Recessive; Heart-hand Syndrome, Slovenian Type; Hutchinson-gilford Progeria Syndrome; Lipodystrophy, Familial Partial, Type 2; Mandibuloacral Dysplasia With Type A Lipodystrophy; Muscular Dystrophy, Congenital, Lmna-related; Muscular Dystrophy, Limb-girdle, Type 1b; Restrictive Dermopathy, Lethal

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