tRNA splicing endonuclease 54 homolog (S. cerevisiae), ELISA Kit

Mouse tRNA-splicing endonuclease subunit Sen54 (TSEN54) ELISA kit; FLJ37147; PCH2A; PCH4; SEN54L; sen54; tRNA splicing endonuclease 54 homolog; tRNA-intron endonuclease Sen54; tRNA splicing endonuclease 54 homolog (S. cerevisiae)

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of TSEN54 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9320702 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tRNA splicing endonuclease 54 homolog (S. cerevisiae) (TSEN54) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TSEN54. The ELISA analytical biochemical technique of the MBS9320702 kit is based on TSEN54 antibody-TSEN54 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TSEN54 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TSEN54. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

59,083 Da

tRNA splicing endonuclease 54 homolog (S. cerevisiae)

tRNA-splicing endonuclease subunit Sen54; tRNA-intron endonuclease Sen54; likely ortholog of H. sapiens SEN54L

tRNA-splicing endonuclease subunit Sen54

Sen54  [Similar Products]

SEN54_MOUSE

TSEN54: Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3' end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3' end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Defects in TSEN54 are the cause of pontocerebellar hypoplasia type 4 (PCH4). Pontocerebellar hypoplasia (PCH) is a heterogeneous group of disorders characterized by an abnormally small cerebellum and brainstem. PCH4 is characterized by severe course and early lethality. Defects in TSEN54 are the cause of pontocerebellar hypoplasia type 2A (PCH2A). Pontocerebellar hypoplasia (PCH) is a heterogeneous group of disorders characterized by an abnormally small cerebellum and brainstem. PCH type 2 is characterized by progressive microcephaly from birth combined with extrapyramidal dyskinesia and chorea, epilepsy, and normal spinal cord findings. Belongs to the SEN54 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus

Cellular Component: nucleus

Biological Process: tRNA splicing; tRNA processing; mRNA processing

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