Product Name
tRNA-splicing endonuclease subunit Sen54 (Tsen54), Recombinant Protein
Full Product Name
Recombinant Mouse tRNA-splicing endonuclease subunit Sen54 (Tsen54)
Product Gene Name
Tsen54 recombinant protein
[Similar Products]
Product Synonym Gene Name
Tsen54[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-525aa, full length protein
Sequence
MEPEPEPGSV EVPAGRVLSA SELRAARSRS QKLPQRSHGP KDFLPDGSEA QAERLRLCRQ ELWQLLAEER VERLGSLVAA EWKPEEGFVE LTSPAGKFWQ TMGYSEEGRQ RLHPEEALYL LECGSIQLFY QDLPLSIQEA YQLLLTEDTL SFLQYQVFSH LKRLGYVVRR FQLSSVVSPY ERQLNLDGYA QCLEDGSGKR KRSSSCRSVN KKPKVLQNSL PPVSLAASSS PACDQSSQYP EEKSQDSSPR QGSELPLQFL GSSEPCSDLA REDVGCDRES HKIENGAKGT PKLRWNFEQI SFPNMASDSR HTFLPAPAPE LLPANVIGRG TDAESWCQKL NQRREKLSRR DREQQAVVQQ FREDVNADPE VRGCSSWQEY KELLQRRQTQ KSQPRPPHLW GQSVTPLLDP DKADCPAAVL QHISVLQTTH LADGGYRLLE KSGGLQISFD VYQADAVATF RKNSPGKPYV RMCISGFDDP VPDLCSLKCL TYQSGDVPLI FALVDHGDIS FYSFRDFTLP RDLGH
3D Structure
ModBase 3D Structure for Q8C2A2
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Tsen54 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for Tsen54. It may not necessarily be applicable to this product.
NCBI Accession #
NP_083833.1
[Other Products]
NCBI GenBank Nucleotide #
NM_029557.1
[Other Products]
UniProt Primary Accession #
Q8C2A2
[Other Products]
UniProt Secondary Accession #
Q9DCI5; B1ATA4; B1ATA5[Other Products]
UniProt Related Accession #
Q8C2A2[Other Products]
Molecular Weight
54,854 Da
NCBI Official Full Name
tRNA-splicing endonuclease subunit Sen54
NCBI Official Synonym Full Names
tRNA splicing endonuclease subunit 54
NCBI Official Symbol
Tsen54 [Similar Products]
NCBI Official Synonym Symbols
0610034P02Rik
[Similar Products]
NCBI Protein Information
tRNA-splicing endonuclease subunit Sen54
UniProt Protein Name
tRNA-splicing endonuclease subunit Sen54
UniProt Synonym Protein Names
tRNA-intron endonuclease Sen54
Protein Family
tRNA-splicing endonuclease
UniProt Gene Name
Tsen54 [Similar Products]
UniProt Synonym Gene Names
Sen54 [Similar Products]
UniProt Comments for Tsen54
Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events ().
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
