Product Name
ANO6, siRNA
Popular Item
Full Product Name
ANO6 siRNA (Mouse)
Product Synonym Names
TMEM16F; Anoctamin-6; Small-conductance calcium-activated nonselective cation channel; SCAN channel; Transmembrane protein 16F
Product Gene Name
ANO6 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q6P9J9
Specificity
ANO6 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ANO6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4°C. Store at -20°C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ANO6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ANO6 sirna
siRNA to inhibit ANO6 expression using RNA interference
Applications Tested/Suitable for ANO6 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ANO6. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001240742.1
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NCBI GenBank Nucleotide #
NM_001253813.1
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UniProt Primary Accession #
Q6P9J9
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UniProt Secondary Accession #
Q8C242[Other Products]
UniProt Related Accession #
Q6P9J9[Other Products]
NCBI Official Full Name
anoctamin-6 isoform 1
NCBI Official Synonym Full Names
anoctamin 6
NCBI Official Symbol
Ano6 [Similar Products]
NCBI Official Synonym Symbols
Tmem16f; AA407480; AW554778; 2900059G15Rik; F730003B03Rik
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NCBI Protein Information
anoctamin-6
UniProt Protein Name
Anoctamin-6
UniProt Synonym Protein Names
Small-conductance calcium-activated nonselective cation channel; SCAN channel; Transmembrane protein 16F
UniProt Gene Name
Ano6 [Similar Products]
UniProt Synonym Gene Names
Tmem16f; SCAN channel [Similar Products]
UniProt Entry Name
ANO6_MOUSE
UniProt Comments for ANO6
ANO6: May act as a calcium-activated chloride channel. It is essential for calcium-dependent exposure of phosphatidylserine on the surface of activated platelets, a process necessary to trigger the clotting system. Defects in ANO6 are the cause of Scott syndrome (SCTS). A mild bleeding disorder due to impaired surface exposure of procoagulant phosphatidylserine (PS) on platelets and other blood cells, following activation with Ca(2+)-elevating agents. Belongs to the anoctamin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter; Membrane protein, multi-pass; Membrane protein, integral; Transporter, ion channel
Cellular Component: cell surface; membrane; integral to membrane; plasma membrane; intracellular
Molecular Function: phospholipid scramblase activity; chloride channel activity; protein homodimerization activity; intracellular calcium activated chloride channel activity; voltage-gated chloride channel activity; voltage-gated ion channel activity; calcium activated cation channel activity
Biological Process: activation of blood coagulation via clotting cascade; transport; dendritic cell chemotaxis; ion transport; phospholipid transport; chloride transport; phospholipid scrambling; lipid transport; blood coagulation; cation transport
Research Articles on ANO6
1. human osteoblasts and osteoblasts from Ano6(-/-) and WT mice, we demonstrate that NCX1 requires Ano6 to efficiently translocate Ca(2+) out of osteoblasts into the calcifying bone matrix
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
