CYCS / Cytochrome c, Monoclonal Antibody

Anti-CYCS / Cytochrome c Antibody (aa1-80, clone 7H8.2C12) IHC-plus; CYCS; CYC; HCS; THC4; Cytochrome c; Cytochrome c; somatic; Pigeon CYCS; Cytochrome c

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2b

7H8.2C12

Mouse

Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C.

Protein G Purified

PBS, 0.2% gelatin, 0.05% sodium azide.

0.5 mg/ml (lot specific)

+4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Small volumes of anti-CYCS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)

ICC, IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Anti-Cytochrome c antibody IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot detection of Cytochrome C in 15 ugs of HeLa cell lysate using antibody at 1:1000. A 15 kD band is detected.

11,749 Da

cytochrome c, somatic

cytochrome c

Cytochrome c

CYC  [Similar Products]

CYC_HUMAN

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]

CYCS: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Belongs to the cytochrome c family.

Protein type: Oxidoreductase; Mitochondrial; Protein phosphatase, Ser/Thr (non-receptor); Apoptosis

Chromosomal Location of Human Ortholog: 7p15.3

Cellular Component: protein phosphatase type 2A complex; mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; cytosol; nucleus

Molecular Function: electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; protein binding; iron ion binding; heme binding; protein serine/threonine phosphatase activity

Biological Process: cellular metabolic process; mitochondrion organization and biogenesis; response to reactive oxygen species; dephosphorylation; apoptosis; organelle organization and biogenesis; cellular respiration; mitochondrial electron transport, cytochrome c to oxygen; DNA fragmentation during apoptosis; caspase activation via cytochrome c; transmembrane transport; mitochondrial electron transport, ubiquinol to cytochrome c

Disease: Thrombocytopenia 4

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