Full Product Name
PJVK siRNA (Mouse)
Product Synonym Names
DFNB59; GM1001; Pejvakin; Autosomal recessive deafness type 59 protein homolog
Product Gene Name
PJVK sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q0ZLH2
Specificity
PJVK siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PJVK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PJVK sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PJVK sirna
siRNA to inhibit PJVK expression using RNA interference
Applications Tested/Suitable for PJVK sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PJVK. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001074180.1
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NCBI GenBank Nucleotide #
NM_001080711.2
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UniProt Primary Accession #
Q0ZLH2
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UniProt Related Accession #
Q0ZLH2[Other Products]
Molecular Weight
39,858 Da
NCBI Official Full Name
pejvakin
NCBI Official Synonym Full Names
deafness, autosomal recessive 59 (human)
NCBI Official Symbol
Dfnb59 [Similar Products]
NCBI Official Synonym Symbols
Pjvk; Gm1001
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NCBI Protein Information
pejvakin
UniProt Protein Name
Pejvakin
UniProt Synonym Protein Names
Autosomal recessive deafness type 59 protein homolog
UniProt Gene Name
Pjvk [Similar Products]
UniProt Synonym Gene Names
Dfnb59; Gm1001 [Similar Products]
UniProt Entry Name
PJVK_MOUSE
UniProt Comments for PJVK
DFNB59: Essential in the activity of auditory pathway neurons. Defects in PJVK are the cause of deafness autosomal recessive type 59 (DFNB59). DFNB59 is a form of sensorineural hearing impairment with absent or severely abnormal auditory brainstem response but normal otoacoustic emissions (auditory neuropathy or auditory dys-synchrony). Auditory neuropathies result from a lesion in the area including the inner hair cells, connections between the inner hair cells and the cochlear branch of the auditory nerve, the auditory nerve itself and auditory pathways of the brainstem. Belongs to the gasdermin family.
Cellular Component: cell soma
Biological Process: sensory perception of sound
Research Articles on PJVK
1. The pejvakin allele described here introduces a premature stop codon, causes outer hair cell defects, and leads to progressive hearing loss.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
