LIM/homeobox protein Lhx3 (LHX3), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of LHX3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7211526 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the LIM/homeobox protein Lhx3 (LHX3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LHX3. The ELISA analytical biochemical technique of the MBS7211526 kit is based on LHX3 antibody-LHX3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LHX3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LHX3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Epigenetics and Nuclear Signaling

43,978 Da

LIM homeobox 3

LIM/homeobox protein Lhx3; LIM homeobox protein 3; LIM/homeodomain protein LHX3

LIM/homeobox protein Lhx3

LIM homeobox protein 3  [Similar Products]

LHX3_HUMAN

This gene encodes a member a large protein family which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor that is required for pituitary development and motor neuron specification. Mutations in this gene cause combined pituitary hormone deficiency 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

LHX3: Acts as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pit-1. Defects in LHX3 are the cause of pituitary hormone deficiency combined type 3 (CPHD3); also known as combined pituitary hormone deficiency with rigid cervical spine or sensorineural deafness with pituitary dwarfism. CPHD is characterized by a complete deficit in all but one (adrenocorticotropin) anterior pituitary hormone and a rigid cervical spine leading to limited head rotation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: transcription factor complex; nucleus

Molecular Function: zinc ion binding; sequence-specific DNA binding

Biological Process: transcription from RNA polymerase II promoter; organ morphogenesis; pituitary gland development; positive regulation of transcription, DNA-dependent; spinal cord motor neuron cell fate specification; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; medial motor column neuron differentiation; motor axon guidance; ventral spinal cord interneuron specification; inner ear development; lung development; negative regulation of apoptosis; placenta development

Disease: Pituitary Hormone Deficiency, Combined, 3

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