Cytochrome c, Monoclonal Antibody

CYC; Cytochrome c

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

Mouse

Recognizes endogenous levels of Cytochrome c protein.

Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Small volumes of anti-CYCS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mouse monoclonal to Cytochrome c

Western Blot (WB), Immunohistochemistry (IHC)

Western Blot: (1/1000 - 1/3000); Immunohistochemistry: (1/200 - 1/500)

Western blot analysis of Cytochrome c expression in Hela (A), 293T (B), NIH3T3 (C), mouse liver (D), rat liver (E), rat kidney (F) whole cell lysates.

Immunohistochemical analysis of Cytochrome c staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

11,749 Da

cytochrome c, somatic

cytochrome c

Cytochrome c

CYC  [Similar Products]

CYC_HUMAN

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]

CYCS: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Belongs to the cytochrome c family.

Protein type: Oxidoreductase; Apoptosis; Mitochondrial; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 7p15.3

Cellular Component: cytosol; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrion; nucleus; protein phosphatase type 2A complex

Molecular Function: electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; heme binding; metal ion binding; protein binding; protein serine/threonine phosphatase activity

Biological Process: apoptosis; caspase activation via cytochrome c; cellular metabolic process; cellular respiration; gene expression; mitochondrial electron transport, cytochrome c to oxygen; mitochondrial electron transport, ubiquinol to cytochrome c; mitochondrion organization and biogenesis; organelle organization and biogenesis; programmed cell death; protein amino acid dephosphorylation; response to reactive oxygen species; transcription initiation from RNA polymerase II promoter

Disease: Thrombocytopenia 4

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