Full Product Name
GNE siRNA (Rat)
Product Synonym Names
GLCNE; Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase
Product Gene Name
GNE sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O35826
Specificity
GNE siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GNE gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GNE sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GNE sirna
siRNA to inhibit GNE expression using RNA interference
Applications Tested/Suitable for GNE sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GNE. It may not necessarily be applicable to this product.
NCBI Accession #
NP_446217.1
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NCBI GenBank Nucleotide #
NM_053765.2
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UniProt Primary Accession #
O35826
[Other Products]
UniProt Related Accession #
O35826[Other Products]
Molecular Weight
79,227 Da
NCBI Official Full Name
bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
NCBI Official Synonym Full Names
glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
NCBI Official Symbol
Gne [Similar Products]
NCBI Official Synonym Symbols
Uae1
[Similar Products]
NCBI Protein Information
bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
UniProt Protein Name
Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
UniProt Synonym Protein Names
UDP-GlcNAc-2-epimerase/ManAc kinaseIncluding the following 2 domains:UDP-N-acetylglucosamine 2-epimerase (hydrolyzing) (EC:3.2.1.183)Alternative name(s):UDP-GlcNAc-2-epimerase; Uridine diphosphate-N-acetylglucosamine-2-epimerase
Protein Family
Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
UniProt Gene Name
Gne [Similar Products]
UniProt Synonym Gene Names
Glcne [Similar Products]
UniProt Entry Name
GLCNE_RAT
NCBI Summary for GNE
bifunctional enzyme, catalyzes the first two steps of biosynthesis of Neu5Ac (UDP-GlcNAc 2-epimerase and ManNAc kinase), precursor of sialic acids [RGD, Feb 2006]
UniProt Comments for GNE
GNE: Regulates and initiates biosynthesis of N- acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development. Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells. Defects in GNE are a cause of sialuria (SIALURIA); also known as sialuria French type. In sialuria, free sialic acid accumulates in the cytoplasm and gram quantities of neuraminic acid are secreted in the urine. The metabolic defect involves lack of feedback inhibition of UDP-GlcNAc 2-epimerase by CMP-Neu5Ac, resulting in constitutive overproduction of free Neu5Ac. Clinical features include variable degrees of developmental delay, coarse facial features and hepatomegaly. Sialuria inheritance is autosomal dominant. Defects in GNE are the cause of inclusion body myopathy type 2 (IBM2). Hereditary inclusion body myopathies are a group of neuromuscular disorders characterized by ***** onset, slowly progressive distal and proximal weakness and a typical muscle pathology including rimmed vacuoles and filamentous inclusions. IBM2 is an autosomal recessive disorder affecting mainly leg muscles, but with an unusual distribution that spares the quadriceps as also observed in Nonaka myopathy. Defects in GNE are the cause of Nonaka myopathy (NM); also known as distal myopathy with rimmed vacuoles (DMRV). NM is an autosomal recessive muscular disorder, allelic to inclusion body myopathy 2. It is characterized by weakness of the anterior compartment of the lower limbs with onset in early *****hood, and sparing of the quadriceps muscles. As the inclusion body myopathy, NM is histologically characterized by the presence of numerous rimmed vacuoles without inflammatory changes in muscle specimens. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Isomerase; Cytoskeletal; Cell adhesion; EC 2.7.1.60; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Motility/polarity/chemotaxis; EC 3.2.1.183; Kinase, other
Cellular Component: cytosol
Molecular Function: protein binding; metal ion binding; UDP-N-acetylglucosamine 2-epimerase activity; ATP binding; N-acylmannosamine kinase activity; hydrolase activity, hydrolyzing O-glycosyl compounds
Biological Process: N-acetylglucosamine biosynthetic process; UDP-N-acetylglucosamine metabolic process; N-acetylneuraminate biosynthetic process; carbohydrate phosphorylation
Research Articles on GNE
1. M712T mutation of GNE gene does not affect GNE oligomerization but affects the solubility and posttranslational modification of the GNE protein.
Precautions
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