Adenosine receptor A2a (ADORA2A), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of ADORA2a. No significant cross-reactivity or interference between ADORA2a and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADORA2a and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ADORA2A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7243419 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adenosine receptor A2a (ADORA2A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADORA2A. The ELISA analytical biochemical technique of the MBS7243419 kit is based on ADORA2A antibody-ADORA2A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADORA2A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADORA2A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: ADORA2a ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADORA2a antibody and an ADORA2a-HRP conjugate. The assay sample and buffer are incubated together with ADORA2a-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADORA2a concentration since ADORA2a from samples and ADORA2a-HRP conjugate compete for the anti-ADORA2a antibody . Since the number of sites is limited, as more sites are occupied by ADORA2a from the sample, fewer sites are left to bind ADORA2a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADORA2a concentration in each sample is interpolated from this standard curve.

Cardiovascular

44,707 Da

adenosine A2a receptor

adenosine receptor A2a; adenosine receptor subtype A2a

Adenosine receptor A2a

ADORA2  [Similar Products]

AA2AR_HUMAN

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013]

ADORA2A: one of several receptor subtypes for adenosine. A G-protein coupled receptor. Activation is mediated by G proteins which activate adenylyl cyclase. Abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: identical protein binding; protein binding; enzyme binding; adenosine receptor activity, G-protein coupled

Biological Process: central nervous system development; nerve growth factor receptor signaling pathway; blood circulation; apoptosis; adenylate cyclase activation; phagocytosis; G-protein signaling, coupled to cAMP nucleotide second messenger; cell-cell signaling; sensory perception; cellular defense response; cAMP biosynthetic process; blood coagulation; inflammatory response; adenosine receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

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