TRIM32, Polyclonal Antibody

72 kda Tat interacting Protein; 72 kDa Tat-interacting protein; BBS11; E3 ubiquitin-protein ligase TRIM32; HT2A; LGMD2H; Limb girdle muscular dystrophy 2H (autosomal recessive); Limb girdle muscular dystrophy 2H; Muscular dystrophy Hutterite type; TAT interactive protein 72KD; TATIP; TRI32_HUMAN; Trim32; Tripartite Motif Containing Protein 32; Tripartite motif-containing protein 32; Zinc finger protein HT2A

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Phospho-TRIM32(Ser339) Antibody detects endogenous levels of TRIM32 only when phosphorylated at Ser339.

Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1mg/ml (lot specific)

Store at -20 degree C. Stable for 12 months from date of receipt.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.

Small volumes of anti-TRIM32 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.

Post Translational Modifications: Ubiquitinated.

Subunit Structure: Interacts with DTNBP1. It self-associates.

Similarity: Belongs to the TRIM/RBCC family.

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA (Peptide): 1:20000-1:40000

At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary

At 1/100 staining mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary

Western blot analysis of Phospho-TRIM32(Ser339) in lysates of 293T?, using Phospho-TRIM32(Ser339) Antibody.

Observed: 72 kDa
Predicted: 72 kDa

tripartite motif containing 32

E3 ubiquitin-protein ligase TRIM32

E3 ubiquitin-protein ligase TRIM32

HT2A  [Similar Products]

TRI32_HUMAN

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes. [provided by RefSeq, Jul 2008]

TRIM32: Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo. Defects in TRIM32 are the cause of limb-girdle muscular dystrophy type 2H (LGMD2H); also known as muscular dystrophy Hutterite type. LGMD2H is an autosomal recessive degenerative myopathy characterized by pelvic girdle, shoulder girdle and quadriceps muscle weakness. Clinical phenotype and severity are highly variable. Disease progression is slow and most patients remain ambulatory into the sixth decade of life. Defects in TRIM32 are the cause of Bardet-Biedl syndrome type 11 (BBS11). Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. A relatively high incidence of BBS is found in the mixed Arab populations of Kuwait and in Bedouin tribes throughout the Middle East, most likely due to the high rate of consaguinity in these populations and a founder effect. Belongs to the TRIM/RBCC family.

Protein type: Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 9q33.1

Cellular Component: cytoplasm; striated muscle thick filament; cytosol; nucleus

Molecular Function: protein binding; ubiquitin binding; protein self-association; myosin binding; zinc ion binding; translation initiation factor binding; RNA binding; Tat protein binding; transcription coactivator activity; ubiquitin-protein ligase activity; ligase activity

Biological Process: fat cell differentiation; regulation of interferon type I production; positive regulation of neurogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein polyubiquitination; positive regulation of proteolysis; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; positive regulation of cell cycle; positive regulation of cell growth; activation of NF-kappaB transcription factor; negative regulation of viral transcription; negative regulation of fibroblast proliferation; positive regulation of interferon type I production; positive regulation of protein catabolic process; innate immune response; positive regulation of transcription factor activity; positive regulation of neuron differentiation; response to UV; positive regulation of cell migration

Disease: Muscular Dystrophy, Limb-girdle, Type 2h; Bardet-biedl Syndrome 11

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