60S acidic ribosomal protein P0, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rat RPLP0. No significant cross-reactivity or interference between Rat RPLP0 and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of RPLP0 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS281972 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 60S acidic ribosomal protein P0 (RPLP0) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RPLP0. The ELISA analytical biochemical technique of the MBS281972 kit is based on RPLP0 antibody-RPLP0 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RPLP0 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RPLP0. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate RPLP0 in samples. An antibody specific for RPLP0 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRPLP0 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RPLP0 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RPLP0 bound in the initial step. The color development is stopped and the intensity of the color is measured.

34,215 Da

ribosomal protein lateral stalk subunit P0

60S acidic ribosomal protein P0

60S acidic ribosomal protein P0

Arbp  [Similar Products]

member of the ribosomal protein family; has similarity to other P ribosomal proteins over the carboxyl terminal sequence [RGD, Feb 2006]

RPLP0: a ribosomal protein of the L10P family. Located in the cytoplasm and nucleus. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit.

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 12q16

Cellular Component: cytoplasm; cytosol; dendrite; endoplasmic reticulum; focal adhesion; intracellular ribonucleoprotein complex; membrane; nucleus; ribosome

Molecular Function: peptide binding; RNA binding; structural constituent of ribosome

Biological Process: lactation; response to selenium ion; ribosome biogenesis

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