tripartite motif-containing 32, ELISA Kit

Human E3 ubiquitin-protein ligase TRIM32 (TRIM32) ELISA kit; RP11-67K19.1; BBS11; HT2A; LGMD2H; TATIP; OTTHUMP00000022763; TAT-interactive protein; 72-KD; zinc-finger protein HT2A; tripartite motif-containing 32

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of TRIM32 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9320353 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tripartite motif-containing 32 (TRIM32) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TRIM32. The ELISA analytical biochemical technique of the MBS9320353 kit is based on TRIM32 antibody-TRIM32 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRIM32 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRIM32. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

71,989 Da

tripartite motif containing 32

E3 ubiquitin-protein ligase TRIM32; zinc finger protein HT2A; zinc-finger protein HT2A; 72 kDa Tat-interacting protein; TAT-interactive protein, 72-KD; tripartite motif-containing 32; tripartite motif-containing protein 32

E3 ubiquitin-protein ligase TRIM32

HT2A  [Similar Products]

TRI32_HUMAN

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes. [provided by RefSeq, Jul 2008]

TRIM32: Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo. Defects in TRIM32 are the cause of limb-girdle muscular dystrophy type 2H (LGMD2H); also known as muscular dystrophy Hutterite type. LGMD2H is an autosomal recessive degenerative myopathy characterized by pelvic girdle, shoulder girdle and quadriceps muscle weakness. Clinical phenotype and severity are highly variable. Disease progression is slow and most patients remain ambulatory into the sixth decade of life. Defects in TRIM32 are the cause of Bardet-Biedl syndrome type 11 (BBS11). Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. A relatively high incidence of BBS is found in the mixed Arab populations of Kuwait and in Bedouin tribes throughout the Middle East, most likely due to the high rate of consaguinity in these populations and a founder effect. Belongs to the TRIM/RBCC family.

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; EC 6.3.2.-; Ligase; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 9q33.1

Cellular Component: cytoplasm; striated muscle thick filament; nucleus; cytosol

Molecular Function: protein binding; ubiquitin binding; protein self-association; myosin binding; zinc ion binding; RNA binding; translation initiation factor binding; Tat protein binding; transcription coactivator activity; ubiquitin-protein ligase activity; ligase activity

Biological Process: fat cell differentiation; regulation of interferon type I production; positive regulation of neurogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein polyubiquitination; positive regulation of proteolysis; protein ubiquitination during ubiquitin-dependent protein catabolic process; positive regulation of cell cycle; protein ubiquitination; positive regulation of cell growth; activation of NF-kappaB transcription factor; negative regulation of viral transcription; negative regulation of fibroblast proliferation; positive regulation of protein catabolic process; positive regulation of interferon type I production; innate immune response; positive regulation of transcription factor activity; positive regulation of neuron differentiation; positive regulation of cell migration; response to UV

Disease: Muscular Dystrophy, Limb-girdle, Type 2h; Bardet-biedl Syndrome 11

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