ARRB2, Polyclonal Antibody

ARRB2; ARB2; ARR2; BARR2; DKFZp686L0365; Beta Arrestin 2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous level of total ARRB2 antibody.

Antibodies were purified by affinity purification using immunogen.

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1.0 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-ARRB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Arrestin proteins function as negative regulators of G protein-coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylated GPCR and the eventual internalization of the receptor and desensitization of GPCR signaling (1). Four distinct mammalian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (X-arrestin) are localized to retinal rods and cones, respectively. Arrestin 2 (also known as b-arrestin 1) and arrestin 3 (b-arrestin 2) are ubiquitously expressed and bind to most GPCRs (2). b-arrestins function as adaptor and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in Erk activation pathways (3,4). b-arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8). Additional evidence suggests that b-arrestins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10).

Total protein Ab

Western Blot (WB), Immunofluorescence (IF)

Western blotting: 1:500 - 1:2000
Immunofluorescence: 1:50 - 1:200

Western blot analysis of RAW264.7 cell lysate using ARRB2 antibody.

Immunofluorescence analysis of U20S cell using ARRB2 antibody. Blue: DAPI for nuclear staining.

44,392 Da

arrestin, beta 2

beta-arrestin-2

Beta-arrestin-2

ARB2; ARR2  [Similar Products]

ARRB2_HUMAN

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

ARRB2: a member of the arrestin/beta-arrestin protein family. These proteins participate in agonist-mediated desensitization of G-protein-coupled receptors. Acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Expressed at high levels in the CNS and peripheral blood leukocytes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: postsynaptic membrane; basolateral plasma membrane; endocytic vesicle; postsynaptic density; cytoplasm; dendritic spine; plasma membrane; coated pit; cytoplasmic vesicle; cytosol; nucleus

Molecular Function: angiotensin receptor binding; protein domain specific binding; protein kinase B binding; type 2A serotonin receptor binding; follicle stimulating hormone receptor binding; alpha-1B adrenergic receptor binding; alpha-1A adrenergic receptor binding; protein binding; enzyme binding; G-protein-coupled receptor binding; ubiquitin protein ligase binding; protein complex binding; mitogen-activated protein kinase binding; type 1 angiotensin receptor binding; protein complex scaffold; D1 dopamine receptor binding; receptor binding

Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; follicle-stimulating hormone signaling pathway; positive regulation of receptor internalization; protein ubiquitination; negative regulation of caspase activity; ***** walking behavior; protein transport; transforming growth factor beta receptor signaling pathway; receptor internalization; negative regulation of interleukin-6 production; detection of temperature stimulus involved in sensory perception of pain; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; platelet activation; Notch signaling pathway; negative regulation of interleukin-12 production; negative regulation of natural killer cell mediated cytotoxicity; positive regulation of synaptic transmission, dopaminergic; negative regulation of tumor necrosis factor production; negative regulation of interleukin-1 beta production; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; G-protein coupled receptor internalization; inhibition of NF-kappaB transcription factor; positive regulation of protein ubiquitination; negative regulation of toll-like receptor signaling pathway; negative regulation of protein ubiquitination; brain development; positive regulation of calcium ion transport; blood coagulation

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