Full Product Name
IDS siRNA (Human)
Product Synonym Names
SIDS; Iduronate 2-sulfatase; Alpha-L-iduronate sulfate sulfatase; Idursulfase
Product Gene Name
IDS sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P22304
Specificity
IDS siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IDS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of IDS sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
IDS sirna
siRNA to inhibit IDS expression using RNA interference
Applications Tested/Suitable for IDS sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for IDS. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000193.1
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NCBI GenBank Nucleotide #
NM_000202.6
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UniProt Primary Accession #
P22304
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UniProt Secondary Accession #
Q14604; Q9BRM3; D3DWT4[Other Products]
UniProt Related Accession #
P22304[Other Products]
Molecular Weight
34,893 Da
NCBI Official Full Name
iduronate 2-sulfatase isoform a preproprotein
NCBI Official Synonym Full Names
iduronate 2-sulfatase
NCBI Official Symbol
IDS [Similar Products]
NCBI Official Synonym Symbols
MPS2; SIDS
[Similar Products]
NCBI Protein Information
iduronate 2-sulfatase
UniProt Protein Name
Iduronate 2-sulfatase
UniProt Synonym Protein Names
Alpha-L-iduronate sulfate sulfatase; Idursulfase
Protein Family
Iduronate 2-sulfatase
UniProt Gene Name
IDS [Similar Products]
UniProt Synonym Gene Names
SIDS; Idursulfase [Similar Products]
UniProt Entry Name
IDS_HUMAN
NCBI Summary for IDS
The protein encoded by this gene belongs to the sulfatase family, is localized to the lysosome, and is involved in lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease, mucopolysaccharidosis type II, also known as Hunter syndrome. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Aug 2013]
UniProt Comments for IDS
IDS: Required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Defects in IDS are the cause of mucopolysaccharidosis type 2 (MPS2); also known as Hunter syndrome. MPS2 is an X-linked lysosomal storage disease characterized by intracellular accumulation of heparan sulfate and dermatan sulfate and their excretion in urine. Most children with MPS2 have a severe form with early somatic abnormalities including skeletal deformities, hepatosplenomegaly, and progressive cardiopulmonary deterioration. A prominent feature is neurological damage that presents as developmental delay and hyperactivity but progresses to mental retardation and dementia. They die before 15 years of age, usually as a result of obstructive airway disease or cardiac failure. In contrast, those with a mild form of MPS2 may survive into *****hood, with attenuated somatic complications and often without mental retardation. Belongs to the sulfatase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; Glycan Metabolism - glycosaminoglycan degradation; EC 3.1.6.13
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: lysosomal lumen
Molecular Function: metal ion binding; iduronate-2-sulfatase activity
Biological Process: chondroitin sulfate metabolic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; chondroitin sulfate catabolic process; pathogenesis
Disease: Mucopolysaccharidosis, Type Ii
Research Articles on IDS
1. p.Ser142Phe and p.Ile360Tyrfs*31 mutations caused the severe disease manifestation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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