Full Product Name
Goat anti-MEFV Antibody
Product Synonym Names
MEFV; Mediterranean fever; HGNC: 6998; FMF; MEF; TRIM20; Mediterranean fever protein; pyrin; MGC126560; MGC126586; marenostrin; MEFV antibody; Mediterranean fever antibody; HGNC: 6998 antibody; FMF antibody; MEF antibody; TRIM20 antibody; Mediterranean fever protein antibody; pyrin antibody; MGC126560 antibody; MGC126586 antibody; marenostrin antibody
Product Gene Name
anti-MEFV antibody
[Similar Products]
Antibody/Peptide Pairs
MEFV peptide (MBS425478) is used for blocking the activity of MEFV antibody (MBS420335)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
EHLKKLRKSG EEQ
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-EHLKKLRKSGEEQ, from the internal region of the protein sequence according to NP_000234.1.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-MEFV antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-MEFV antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-MEFV antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 80kDa band observed in Human Peripheral Blood Leukocyte lysate. Recommended concentration: 1-3ug/ml during overnight incubation.
NCBI/Uniprot data below describe general gene information for MEFV. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000234.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000243.2
[Other Products]
UniProt Secondary Accession #
Q3MJ84; Q96PN4; Q96PN5; D3DUC0; F5H0Q3[Other Products]
UniProt Related Accession #
O15553[Other Products]
Molecular Weight
50,837 Da
NCBI Official Full Name
pyrin isoform 1
NCBI Official Synonym Full Names
Mediterranean fever
NCBI Official Symbol
MEFV [Similar Products]
NCBI Official Synonym Symbols
FMF; MEF; TRIM20
[Similar Products]
NCBI Protein Information
pyrin
UniProt Protein Name
Pyrin
UniProt Synonym Protein Names
Marenostrin
UniProt Gene Name
MEFV [Similar Products]
UniProt Synonym Gene Names
[Similar Products]
UniProt Entry Name
MEFV_HUMAN
NCBI Summary for MEFV
This gene encodes a protein, also known as pyrin or marenostrin, that is an important modulator of innate immunity. Mutations in this gene are associated with Mediterranean fever, a hereditary periodic fever syndrome. [provided by RefSeq, Jul 2008]
UniProt Comments for MEFV
pyrin: Probably controls the inflammatory response in myelomonocytic cells at the level of the cytoskeleton organization. Defects in MEFV are the cause of familial Mediterranean fever autosomal recessive (ARFMF). ARFMF is an inherited disorder characterized by recurrent episodic fever, serosal inflammation and pain in the abdomen, chest or joints. ARFMF is frequently complicated by amyloidosis, which leads to renal failure and can be prophylactically treated with colchicine. ARFMF primarily affects ancestral ethnic groups living around the Mediterranean basin: North African Jews, Armenians, Arabs and Turks. The disease is also distributed in other populations including Greeks, Cypriots, Italians and Spanish, although at a lower prevalence. Defects in MEFV are the cause of familial Mediterranean fever autosomal dominant (ADFMF). ADFMF is characterized by periodic fever, serosal inflammation and pain in the abdomen, chest or joints as seen also in the autosomal recessive form of the disease. It is associated with renal amyloidosis and characterized by colchicine unresponsiveness. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: autophagic vacuole; cytoplasm; cytoplasmic vesicle; cytosol; lamellipodium; microtubule; microtubule associated complex; nucleus; ruffle
Molecular Function: actin binding; protein binding; zinc ion binding
Biological Process: inflammatory response; negative regulation of inflammatory response; negative regulation of interleukin-1 beta production; negative regulation of interleukin-12 production; positive regulation of autophagy
Disease: Familial Mediterranean Fever; Familial Mediterranean Fever, Autosomal Dominant
Research Articles on MEFV
1. MEFV mutations M694V and M680I are associated with Behcet's disease. [meta-analysis]
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
