BDNF/NT-3 growth factors receptor, Recombinant Protein

GP145-TrkB; trk-B; TRKB; NTRK2

For Research Use Only. Not for use in diagnostic procedures.

Baculovirus

> 95% by SDS-PAGE

Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.

0.5 mg/ml (determined by Absorbance at 280nm) (lot specific)

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of BDNF/NT-3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

NTRK2, also known as BDNF/NT-3 growth factors receptor, is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. It plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. Recombinant human NTRK2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Neuroscience

SDS-PAGE

45.2kDa (407aa) 57-70kDa (SDS-PAGE under reducing conditions)

neurotrophic receptor tyrosine kinase 2

BDNF/NT-3 growth factors receptor

BDNF/NT-3 growth factors receptor

TRKB; Trk-B  [Similar Products]

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.

Yeo GS., et al. (2004) Nat Neurosci. 7:1187-1189.; Banfield MJ., et al. (2001) Structure. 9:1191-1199.

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