Product Name
COL4A5, Polyclonal Antibody
Full Product Name
COL4A5 Polyclonal Antibody
Product Synonym Names
Arresten; CO4A1_HUMAN; COL4A1; COL4A1 NC1 domain; COL4A2; COL4A3; COL4A4; COL4A5; collagen alpha-1(IV) chain; Collagen IV Alpha 1 Polypeptide; Collagen IV Alpha 2 Polypeptide; Collagen Of Basement Membrane Alpha 1 Chain; Collagen Of Basement Membrane Alpha 2 Chain; Collagen Type IV Alpha 1; Collagen Type IV Alpha 2; Collagen Type IV Alpha 3; Collagen Type IV Alpha 4; Collagen Type IV Alpha 5; Collagen Type IV
Product Gene Name
anti-COL4A5 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P29400
Purity/Purification
Antigen affinity purification
Concentration
1mg/ml (lot specific)
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Immunogen
Recombinant protein of human COL4A5
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-11360
Preparation and Storage
Store at -20 degree C. Avoid freeze/ thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-COL4A5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-COL4A5 antibody
Western Blot (WB)
Application Notes for anti-COL4A5 antibody
Western Blot: 1:500 - 1:2000
Western Blot (WB) of anti-COL4A5 antibody
Western blot analysis of extracts of various cells, using COL4A5 antibody.
NCBI/Uniprot data below describe general gene information for COL4A5. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000486.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000495.4
[Other Products]
UniProt Primary Accession #
P29400
[Other Products]
UniProt Secondary Accession #
Q16006; Q16126; Q6LD84; Q7Z700; Q9NUB7[Other Products]
UniProt Related Accession #
P29400[Other Products]
Molecular Weight
161,632 Da
NCBI Official Full Name
collagen alpha-5(IV) chain isoform 1
NCBI Official Synonym Full Names
collagen type IV alpha 5 chain
NCBI Official Symbol
COL4A5 [Similar Products]
NCBI Official Synonym Symbols
ATS; ASLN; CA54
[Similar Products]
NCBI Protein Information
collagen alpha-5(IV) chain
UniProt Protein Name
Collagen alpha-5(IV) chain
UniProt Gene Name
COL4A5 [Similar Products]
UniProt Entry Name
CO4A5_HUMAN
NCBI Summary for COL4A5
This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]
UniProt Comments for COL4A5
COL4A5: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X- linked (APSX). APSX is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis; Extracellular matrix
Chromosomal Location of Human Ortholog: Xq22
Cellular Component: endoplasmic reticulum lumen; extracellular region
Biological Process: collagen catabolic process; extracellular matrix organization and biogenesis
Disease: Alport Syndrome, X-linked
Research Articles on COL4A5
1. alpha5(IV), but not alpha1(IV), promotes lung cancer cell proliferation and tumor angiogenesis through non-integrin collagen receptor DDR1-mediated ERK activation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
