acyl-Coenzyme A oxidase 1, palmitoyl, ELISA Kit

Mouse Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1) ELISA kit; ACOX; MGC1198; PALMCOX; SCOX; acyl-CoA oxidase; straight-chain; acyl-Coenzyme A oxidase 1; peroxisomal fatty acyl-CoA oxidase; acyl-Coenzyme A oxidase 1; palmitoyl

For Research Use Only. Not for use in diagnostic procedures.

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ACOX1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9316691 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the acyl-Coenzyme A oxidase 1, palmitoyl (ACOX1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACOX1. The ELISA analytical biochemical technique of the MBS9316691 kit is based on ACOX1 antibody-ACOX1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACOX1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACOX1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of ACOX1 (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

74,649 Da

acyl-Coenzyme A oxidase 1, palmitoyl

peroxisomal acyl-coenzyme A oxidase 1; palmitoyl-CoA oxidase; peroxisomal fatty acyl-coenzyme A oxidase; peroxisomal straight-chain Acyl-CoA oxidase

Peroxisomal acyl-coenzyme A oxidase 1

Acox; Paox; AOX  [Similar Products]

ACOX1_MOUSE

This gene encodes a member of the acyl-coenzyme A oxidase family. The encoded protein is localized to peroxisomes and is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-coenzyme A to 2-trans-enoyl-coenzyme A. Disruption of this gene results in microvesicular steatohepatitis, spontaneous peroxisome proliferation, and the eventual development of hepatocellular carcinomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]

ACOX1: Catalyzes the desaturation of acyl-CoAs to 2-trans- enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy- palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl- CoA. Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD); also known as peroxisomal acyl-CoA oxidase deficiency. Pseudo-NALD is a peroxisomal single-enzyme disorder. Clinical features include mental retardation, leukodystrophy, seizures, mild hepatomegaly, hearing deficit. Pseudo-NALD is characterized by increased plasma levels of very-long chain fatty cids, due to decreased or absent peroxisome acyl-CoA oxidase activity. Peroxisomes are intact and functioning. Belongs to the acyl-CoA oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.3.3.6; Oxidoreductase; Lipid Metabolism - fatty acid; Lipid Metabolism - unsaturated fatty acid biosynthesis; Lipid Metabolism - alpha-linolenic acid

Cellular Component: nucleoplasm; peroxisomal membrane; membrane; intracellular membrane-bound organelle; mitochondrion; cytoplasm; plasma membrane; nucleolus; peroxisome; nucleus

Molecular Function: palmitoyl-CoA oxidase activity; acyl-CoA dehydrogenase activity; acyl-CoA oxidase activity; FAD binding; oxidoreductase activity, acting on the CH-CH group of donors; protein N-terminus binding; oxidoreductase activity; fatty acid binding; PDZ domain binding; receptor binding

Biological Process: peroxisome fission; fatty acid beta-oxidation using acyl-CoA oxidase; generation of precursor metabolites and energy; fatty acid beta-oxidation; very-long-chain fatty acid metabolic process; metabolic process; lipid homeostasis; spermatogenesis; fatty acid oxidation; lipid metabolic process; fatty acid metabolic process; prostaglandin metabolic process

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.