Product Name
MUTYH / MYH, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human MUTYH / MYH
Product Synonym Names
Anti-MUTYH / MYH Antibody (aa531-546) IHC-plus; MUTYH; HMYH; MYH; MutY (E Coli) homolog; CYP2C; MutY homolog; MutY homolog (E Coli); Human MUTYH; MYH
Product Gene Name
anti-MUTYH antibody
[Similar Products]
Product Synonym Gene Name
MYH[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9UIF7
Specificity
A synthetic peptide corresponding to amino acids 531-546 of human MYH.
Purity/Purification
Protein G Purified
Form/Format
PBS, 0.2% gelatin, 0.05% sodium azide.
Concentration
0.5 mg/ml (lot specific)
Immunogen Description
Synthetic peptide from human MUTYH.
Immunogen Type
Synthetic peptide
Immunogen
MUTYH / MYH antibody was raised against synthetic peptide from human MUTYH.
Antigen Modification
aa531-546
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Other Notes
Small volumes of anti-MUTYH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-MUTYH antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-MUTYH antibody
IHC-P (10 ug/ml), WB (1 - 2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Immunohistochemistry (IHC) of anti-MUTYH antibody
Anti-MUTYH antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Western Blot (WB) of anti-MUTYH antibody
Western blot of MYH in 20 ugs of HeLa cell lysate using antibody at 2 ug/ml.
NCBI/Uniprot data below describe general gene information for MUTYH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036354.1
[Other Products]
NCBI GenBank Nucleotide #
NM_012222.2
[Other Products]
UniProt Primary Accession #
Q9UIF7
[Other Products]
UniProt Secondary Accession #
Q15830; Q9UBP2; Q9UBS7; Q9UIF4; Q9UIF5; Q9UIF6; D3DPZ4[Other Products]
UniProt Related Accession #
Q9UIF7[Other Products]
Molecular Weight
57,446 Da
NCBI Official Full Name
A/G-specific adenine DNA glycosylase isoform 1
NCBI Official Synonym Full Names
mutY homolog
NCBI Official Symbol
MUTYH [Similar Products]
NCBI Official Synonym Symbols
MYH
[Similar Products]
NCBI Protein Information
A/G-specific adenine DNA glycosylase
UniProt Protein Name
A/G-specific adenine DNA glycosylase
UniProt Synonym Protein Names
MutY homolog; hMYH
UniProt Gene Name
MUTYH [Similar Products]
UniProt Synonym Gene Names
MYH; hMYH [Similar Products]
UniProt Entry Name
MUTYH_HUMAN
NCBI Summary for MUTYH
This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for MUTYH
MUTYH: Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2- OH-A DNA glycosylase activities. Belongs to the Nth/MutY family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA repair, damage; Tumor suppressor; EC 3.2.2.-; Mitochondrial; Hydrolase
Chromosomal Location of Human Ortholog: 1p34.1
Cellular Component: nucleoplasm; mitochondrion; nucleus
Molecular Function: protein binding; 4 iron, 4 sulfur cluster binding; metal ion binding; hydrolase activity, acting on glycosyl bonds; MutLalpha complex binding; MutSbeta complex binding; MutLbeta complex binding; MutSalpha complex binding
Biological Process: mismatch repair; depurination; base-excision repair, AP site formation; base-excision repair; DNA repair
Disease: Gastric Cancer; Pilomatrixoma; Familial Adenomatous Polyposis, 2
Research Articles on MUTYH
1. A heterozygous p.Arg19 * MUTYH variant and a heterozygous p.Arg109Trp MUTYH variant were detected in one patient each.
Precautions
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Disclaimer
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