ATP2A2/SERCA2, Polyclonal Antibody

ATP2A2; ATP2A2; ATP2B; DAR; DD; DKFZp686P0211; FLJ20293; FLJ38063; MGC45367; SERCA2 PROPERTIES

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 12; NC_000012.11 (110719032..110788898). Location: 12q24.11

Polyclonal

IgG

Rabbit

Affinity purification

Liquid

Upon delivery aliquot and store at -20 degree C or -80 degree C.

Small volumes of anti-ATP2A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rabbit polyclonal to ATP2A2/SERCA2

The ATP2A2 (SERCA2) calcium pump is one of several sarcoplasmic and endoplasmic reticulum Ca2+-ATPases responsible for regulating calcium transport across intracellular membranes (1). Multiple isoforms have been isolated, with ATP2A2a (SERCA2a) found predominantly in the sarcoplasmic reticulum of muscle cells and ATP2A2b (SERCA2b) more ubiquitously expressed in the endoplasmic reticulum of most cell types (2). An isoform containing a truncated carboxy region (ATP2A2c) is expressed in epithelial and hematopoietic cell lines and may be involved in monocyte differentiation (3). Post-translational modification of ATP2A2 (SERCA2), including phosphorylation and tyrosine nitration, modify Ca2+ -ATPase activity and calcium transport (4,5). Mutation in the corresponding ATP2A2 (SERCA2) gene results in Darier disease, a skin disorder characterized by the presence of dark, keratotic papules or rash found on the head and torso (6).

Western Blot (WB)

Western Blot (WB): 1/500-1000

Western blot analysis of extracts of various cell lines, using ATP2A2 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

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1. Vangheluwe, P. et al. (2005) Cell Calcium 38, 291-302. 2. de Smedt, H. et al. (1991) J Biol Chem 266, 7092-5. 3. Gélébart, P. et al. (2003) Biochem Biophys Res Commun 303, 676-84. 4. Hawkins, C. et al. (1995) Mol Cell Biochem 142, 131-8. 5. Viner, R.I. et al. (1999) Biochem J 340 (Pt 3), 657-69. 6. Sakuntabhai, A. et al. (1999) Hum Mol Genet 8, 1611-9.

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