ATP2A2/SERCA2, Polyclonal Antibody

Goat Polyclonal to Human ATP2A2/SERCA2; ATP2A2; ATP2B; Calcium pump 2; DAR; SR Ca(2+)-ATPase 2; Cardiac Ca2+ ATPase; DD; SERCA2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Human ATP2A2/SERCA2. This antibody is expected to recognize both reported isoforms (NP_001672.1; NP_733765.1).

Immunoaffinity Purified

Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

Store at -20 degree C.
Avoid freeze-thaw cycles.

Small volumes of anti-ATP2A2/SERCA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

ATP2a2 (Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 ATPase), also known as SERCA2 (Sarcoplasmic reticulum Ca(2+)-ATPase 2), belongs to the large family of P-type cation pumps that couple ATP hydrolysis withcation transport across membranes. the ATP2a2 pump maintains low cytosolic Ca(2+) concentrations by actively transporting Ca(2+) from the cytosol into the sarco/endoplasmic reticulum lumen.

Transporter; ATPase - P type, type IIA

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

ELISA: 1:128000
IHC-P: 5 ug/ml
WB: 0.1-0.3 ug/ml

Anti-ATP2A2/SERCA2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

ATP2A2 antibody (0.1 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

109,734 Da

ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2

sarcoplasmic/endoplasmic reticulum calcium ATPase 2

Sarcoplasmic/endoplasmic reticulum calcium ATPase 2

ATP2B; SERCA2; SR Ca(2+)-ATPase 2  [Similar Products]

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle (PubMed:16402920). Acts as a regulator of TNFSF11-mediated Ca2+ signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca (2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca (2+) signaling cascades that promote osteoclast differentiation and activation ().

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