DGKQ, Polyclonal Antibody

Diacylglycerol kinase theta; DAG kinase theta; Diglyceride kinase theta; DGK-theta; DGKQ; DAGK4

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This DGKQ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 912-942 amino acids from the C-terminal region of human DGKQ.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-DGKQ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Diacylglycerol (DAG) is an allosteric activator of protein kinase C. DAG also participates in regulating RAS and RHO family proteins by activating the guanine nucleotide exchange factors VAV and RASGRP1. DAG is also involved in the synthesis of phospholipids and triacylglycerols. Tight regulation of DAG levels is achieved via DAG kinases (DGKs), which remove DAG by phosphorylate it to phosphatidic acid.The predicted 882-amino acid human DGKQ (DGK-theta) protein is 90% identical in sequence to the rat homolog. DGK-theta is comprised of a pleckstrin homology domain and the conserved DGK putative catalytic domain. In contrast to other DGK isotypes, DGK-theta contains 3 rather than 2 cysteine-rich zinc-binding domains, an N-terminal proline- and glycine-rich region, and a RAS-associating domain. Highest tissue expression in the rat is in the brain. Defects in eye-specific DAGK genes cause retinal degeneration in Drosophila; thus, DAGK genes are candidates for human eye disease.

Signal Transduction

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

WB~~1:1000

DGKQ Antibody (R927) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DGKQ antibody detected the DGKQ protein (arrow).

DGKQ Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DGKQ Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

DGKQ Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

101155

diacylglycerol kinase, theta 110kDa

diacylglycerol kinase theta

Diacylglycerol kinase theta

DAGK4; DAG kinase theta; DGK-theta  [Similar Products]

DGKQ_HUMAN

The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq, Jul 2008]

DGKQ: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor. May be involved in cAMP- induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity. Belongs to the eukaryotic diacylglycerol kinase family.

Protein type: Motility/polarity/chemotaxis; Lipid Metabolism - glycerolipid; EC 2.7.1.107; Lipid Metabolism - glycerophospholipid; Kinase, lipid

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: cytoskeleton; cytoplasm; plasma membrane; nuclear speck; cytosol; nucleus

Molecular Function: protein binding; phospholipase binding; transcription activator binding; metal ion binding; kinase binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; G-protein coupled receptor protein signaling pathway; platelet activation; cAMP-mediated signaling; protein kinase C activation; blood coagulation; phosphorylation; response to ATP

Pilz, A., et al., Genomics 26(3):599-601 (1995).

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