Anoctamin 6 (ANO6), ELISA Kit

TMEM16F; SCAN channel; Transmembrane Protein 16F; Small-conductance calcium-activated nonselective cation channel

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Anoctamin 6 (ANO6).
No significant cross-reactivity or interference between Anoctamin 6 (ANO6) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ANO6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2023354 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anoctamin 6 (ANO6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ANO6. The ELISA analytical biochemical technique of the MBS2023354 kit is based on ANO6 antibody-ANO6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANO6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANO6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anoctamin 6 (ANO6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Anoctamin 6 (ANO6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anoctamin 6 (ANO6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anoctamin 6 (ANO6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Tumor immunity

108,102 Da

anoctamin 6

anoctamin-6

Anoctamin-6

TMEM16F; SCAN channel  [Similar Products]

ANO6_HUMAN

This gene encodes a multi-pass transmembrane protein that belongs to the anoctamin family. This protein is an essential component for the calcium-dependent exposure of phosphatidylserine on the cell surface. The scrambling of phospholipid occurs in various biological systems, such as when blood platelets are activated, they expose phosphatidylserine to trigger the clotting system. Mutations in this gene are associated with Scott syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

ANO6: May act as a calcium-activated chloride channel. It is essential for calcium-dependent exposure of phosphatidylserine on the surface of activated platelets, a process necessary to trigger the clotting system. Defects in ANO6 are the cause of Scott syndrome (SCTS). A mild bleeding disorder due to impaired surface exposure of procoagulant phosphatidylserine (PS) on platelets and other blood cells, following activation with Ca(2+)-elevating agents. Belongs to the anoctamin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter; Membrane protein, integral; Transporter, ion channel

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: cell surface; membrane; plasma membrane; intracellular

Molecular Function: phospholipid scramblase activity; protein homodimerization activity; intracellular calcium activated chloride channel activity; voltage-gated ion channel activity; voltage-gated chloride channel activity; calcium activated cation channel activity

Biological Process: activation of blood coagulation via clotting cascade; dendritic cell chemotaxis; chloride transport; phospholipid scrambling; blood coagulation; lipid transport; transmembrane transport; cation transport

Disease: Scott Syndrome

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