Product Name
GTF2I, siRNA
Full Product Name
GTF2I siRNA (Human)
Product Synonym Names
BAP135; WBSCR6; General transcription factor II-I; GTFII-I; TFII-I; Bruton tyrosine kinase-associated protein 135; BAP-135; BTK-associated protein 135; SRF-Phox1-interacting protein; SPIN; Williams-Beuren syndrome chromosomal region 6 protein
Product Gene Name
GTF2I sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P78347
Specificity
GTF2I siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GTF2I gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GTF2I sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GTF2I sirna
siRNA to inhibit GTF2I expression using RNA interference
Applications Tested/Suitable for GTF2I sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GTF2I. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001157108.1
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NCBI GenBank Nucleotide #
NM_001163636.2
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UniProt Primary Accession #
P78347
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UniProt Secondary Accession #
O14743; O15359; O43546; O43588; O43589; Q75M85; Q75M86; Q75M87; Q75M88; Q86U51; Q9BSZ4[Other Products]
UniProt Related Accession #
P78347[Other Products]
Molecular Weight
30,409 Da
NCBI Official Full Name
general transcription factor II-I isoform 5
NCBI Official Synonym Full Names
general transcription factor IIi
NCBI Official Symbol
GTF2I [Similar Products]
NCBI Official Synonym Symbols
WBS; DIWS; SPIN; IB291; BAP135; BTKAP1; TFII-I; WBSCR6; GTFII-I
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NCBI Protein Information
general transcription factor II-I
UniProt Protein Name
General transcription factor II-I
UniProt Synonym Protein Names
Bruton tyrosine kinase-associated protein 135; BAP-135; BTK-associated protein 135; SRF-Phox1-interacting protein; SPIN; Williams-Beuren syndrome chromosomal region 6 protein
Protein Family
General transcription factor
UniProt Gene Name
GTF2I [Similar Products]
UniProt Synonym Gene Names
BAP135; WBSCR6; GTFII-I; TFII-I; BAP-135; BTK-associated protein 135; SPIN [Similar Products]
UniProt Entry Name
GTF2I_HUMAN
NCBI Summary for GTF2I
This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]
UniProt Comments for GTF2I
GTF2I: a multifunctional phosphoprotein with roles in transcription and signal transduction. Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and Phox1 and interacts cooperatively with Phox1 to promote serum-inducible transcription of a reporter gene driven by the C- FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Its gene is known to contain at least 34 exons. Four splice variant isoforms have been described.
Protein type: Transcription, coactivator/corepressor; DNA-binding
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: nucleoplasm; cell projection; cell soma; membrane; cytoplasm; nucleus
Molecular Function: protein binding; DNA binding; mitogen-activated protein kinase binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; negative regulation of angiogenesis; regulation of transcription, DNA-dependent; reduction of cytosolic calcium ion concentration; transition between slow and fast fiber; signal transduction
Disease: Williams-beuren Syndrome
Research Articles on GTF2I
1. Findings implicate the GTF2I gene in the neurogenetic basis of social communication and social anxiety, both in Williams syndrome and among individuals in healthy populations
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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