Full Product Name
FOXD3 (Forkhead Box D3, HFH2, HNF-3 Forkhead Transcription Factor Genesis)
Product Synonym Names
Anti -FOXD3 (Forkhead Box D3, HFH2, HNF-3 Forkhead Transcription Factor Genesis)
Product Gene Name
anti-FOXD3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000001.10 (63788730..63790797). Location: 1p31.3
3D Structure
ModBase 3D Structure for Q9UJU5
Species Reactivity
Human, Mouse
Specificity
Recognizes endogenous levels of human FOXD3. Species Crossreactivity: mouse.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Immunogen
Synthetic peptide corresponding to human FOXD3.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-FOXD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-FOXD3 antibody
Embryonic stem cells require the forkhead transcriptional regulator FoxD3 for survival. Following gastrulation, FoxD3 generally gets downregulated, except in the neural crest. A variety of growth factors induce FoxD3 expression, including FGF8 and SNAIL, maintaining the effected cells in an undifferentiated state. Thus defects in FoxD3 induction may cause premature differentiation and/or migration-asociated birth defects.
Product Categories/Family for anti-FOXD3 antibody
Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-FOXD3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-FOXD3 antibody
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot (HUVEC cells): 1:500-1:1000
Immunohistochemistry (Paraffin): 1:50-1:200
NCBI/Uniprot data below describe general gene information for FOXD3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036315.1
[Other Products]
NCBI GenBank Nucleotide #
NM_012183.2
[Other Products]
UniProt Primary Accession #
Q9UJU5
[Other Products]
UniProt Secondary Accession #
Q9BYM2; Q9UDD1[Other Products]
UniProt Related Accession #
Q9UJU5[Other Products]
Molecular Weight
47,630 Da[Similar Products]
NCBI Official Full Name
forkhead box protein D3
NCBI Official Synonym Full Names
forkhead box D3
NCBI Official Symbol
FOXD3 [Similar Products]
NCBI Official Synonym Symbols
AIS1; HFH2; Genesis
[Similar Products]
NCBI Protein Information
forkhead box protein D3; OTTHUMP00000010713; HNF3/FH transcription factor genesis
UniProt Protein Name
Forkhead box protein D3
UniProt Synonym Protein Names
HNF3/FH transcription factor genesis
Protein Family
Forkhead box protein
UniProt Gene Name
FOXD3 [Similar Products]
UniProt Synonym Gene Names
HFH2 [Similar Products]
UniProt Entry Name
FOXD3_HUMAN
NCBI Summary for FOXD3
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. Mutations in this gene cause autoimmune susceptibility 1. [provided by RefSeq]
UniProt Comments for FOXD3
FOXD3: Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis. Expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 1p31.3
Cellular Component: nucleoplasm; nuclear chromatin
Molecular Function: protein binding; double-stranded DNA binding; transcription factor activity
Biological Process: embryonic placenta development; transcription, DNA-dependent; somatic stem cell maintenance; in utero embryonic development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; trophectodermal cell differentiation
Disease: Autoimmune Disease, Susceptibility To, 1
Research Articles on FOXD3
1. Studies show FOXD3 is suppressed by B-RAF, uncover a novel role and mechanism for FOXD3 as a negative cell cycle regulator, and have implications for the repression of melanocytic lineage cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
