IRAK1BP1, Polyclonal Antibody

AIP70; SIMPL

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total IRAK1BP1 protein.

Antigen affinity purification.

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

1.9 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-IRAK1BP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

SIMPL, also known as IRAK1BP1 (interleukin-1 receptor-associated kinase 1-binding protein 1), is a 260 amino acid protein belonging to the IRAK1BP1 family. In addition to containing an intrinsically disordered region which associates with HSPA1 and HSPA8, SIMPL also interacts directly with IRAK1 and promotes RELA transcriptional activity. SIMPL is a major component of the IRAK1-dependent TNFRSF1A signaling pathway in which TNF-alpha induces NF-kappa-B gene transcription, leading to an inhibition of proinflammatory cytokines. Inactivation of SIMPL inhibits tumor necrosis factor receptor type I-induced NF-kappaB activity. Localizing to the nucleus and cytoplasm, SIMPL requires the phosphorylation of Ser-56 and/or Ser-62 to be fully active.

Total protein Ab

Western Blot (WB), Immunohistochemistry (IHC)

Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200

Gel: 10%SDS-PAGE Lysates (from left to right): Human fetal liver tissue and mouse heart tissue,human testis tissue Amount of lysate: 40ug per lane Primary antibody: 1/475 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using at dilution 1/40.

29,106 Da

interleukin-1 receptor-associated kinase 1 binding protein 1

interleukin-1 receptor-associated kinase 1-binding protein 1

Interleukin-1 receptor-associated kinase 1-binding protein 1

IRAK1-binding protein 1  [Similar Products]

IKBP1_HUMAN

IRAK1BP1: Component of the IRAK1-dependent TNFRSF1A signaling pathway that leads to NF-kappa-B activation and is required for cell survival. Acts by enhancing RELA transcriptional activity. Belongs to the IRAK1BP1 family.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 6q14-q15

Cellular Component: cytoplasm; nucleus

Biological Process: I-kappaB kinase/NF-kappaB cascade; regulation of transcription, DNA-dependent; transcription, DNA-dependent; immune response

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