HEY2, Polyclonal Antibody

Hairy/enhancer-of-split related with YRPW motif protein 2; Cardiovascular helix-loop-helix factor 1; hCHF1; Hairy and enhancer of split-related protein 2; HESR-2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total HEY2 protein.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1.0 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-HEY2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.Chin M.T., J. Biol. Chem. 275:6381-6387(2000).Nakagawa O., Proc. Natl. Acad. Sci. U.S.A. 97:13655-13660(2000).Iso T., Mol. Cell. Biol. 21:6071-6079(2001).

Total protein Ab

Western Blot (WB)

Western blotting: 1:500~1:3000

Western blot analysis of extracts from K562 cells, using HEY2 antibody.

35,808 Da

hes-related family bHLH transcription factor with YRPW motif 2

hairy/enhancer-of-split related with YRPW motif protein 2

Hairy/enhancer-of-split related with YRPW motif protein 2

BHLHB32; CHF1; GRL; HERP; HERP1; HRT2; hCHF1; bHLHb32; HESR-2; HRT-2; hHRT2  [Similar Products]

HEY2_HUMAN

This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The encoded protein forms homo- or hetero-dimers that localize to the nucleus and interact with a histone deacetylase complex to repress transcription. Expression of this gene is induced by the Notch signal transduction pathway. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

HEY2: Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'- CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6. Belongs to the HEY family.

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; Sin3 complex; transcriptional repressor complex; cytoplasm; nucleus

Molecular Function: protein dimerization activity; protein binding; sequence-specific DNA binding; histone deacetylase binding; transcription factor activity; transcription factor binding

Biological Process: cardiac muscle hypertrophy; transcription from RNA polymerase II promoter; Notch signaling pathway; cell fate commitment; ventricular cardiac muscle cell development; positive regulation of heart rate; negative regulation of transcription from RNA polymerase II promoter; protein-DNA complex assembly; regulation of auditory receptor cell differentiation; negative regulation of Notch signaling pathway; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; vasculogenesis; anterior/posterior axis specification; positive regulation of cardiac muscle cell proliferation; mesenchymal cell development

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