Nav1.8, Monoclonal Antibody

PN3; Na(V)1.8; Scn10a

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a

S134-12

Mouse

Protein G Purified

1mg/mL (lot specific)

-20 degree C

Small volumes of anti-Scn10a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background Info: Detects ~220kDa. No cross reactivity against other Nav channels

Scientific Background: Nav1.8 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1.8 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1.8 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals (3).

Ion Channels, Neuroscience

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134-12. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic staining and some dull nuclear staining.

Western Blot analysis of Monkey COS transient cell lysate showing detection of Nav1.8 protein using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134-12. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134-12. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Heavy filaggrin-like staining, lower epidermal cells have some staining.

219,605 Da[Similar Products]

sodium channel, voltage-gated, type X, alpha subunit

sodium channel protein type 10 subunit alpha; sensory neuron sodium channel; peripheral nerve sodium channel 3; sodium channel type X alpha polypeptide; sodium channel protein type X subunit alpha; voltage-gated sodium channel subunit alpha Nav1.8; sodium channel voltage-gated type X alpha polypeptide; sodium channel, voltage-gated, type 10, alpha polypeptide

Sodium channel protein type 10 subunit alpha

Sns; PN3  [Similar Products]

SCNAA_RAT

voltage-gated sodium channel that is resistant to tetrodotoxin [RGD, Feb 2006]

SCN10A: This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant sodium channel isoform. Its electrophysiological properties vary depending on the type of the associated beta subunits (in vitro). Plays a role in neuropathic pain mechanisms. Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.

Protein type: Membrane protein, integral; Channel, sodium; Membrane protein, multi-pass

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Dray A. (2008) Br. J. Anaesth. 101(1): 48-58. 4. Dray A., Read S.J (2007) Arthritis Res. Ther. 9(3): 212. 5. Samuels M.E., teMorshe R.H., Lynch M.E., Drenth J.P. (2008) Mol Pain. 4: 21.

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