Product Name
Collagen Type IV (COL4), DIY ELISA Kit
Full Product Name
Collagen Type IV (COL4) ELISA Kit DIY Materials
Product Synonym Names
Type-IV Collagen
Product Gene Name
COL4 diy elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Collagen Type IV (COL4). No significant cross-reactivity or interference between Collagen Type IV (COL4) and analogues was observed.
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other
biological fluids.
Detection Range
7.8-500ng/mL
Reagent Contents
Capture
Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customerâs specifications, please inquire.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of COL4 diy elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for COL4 diy elisa kit
Tumor immunity; Hepatology
Application Notes for COL4 diy elisa kit
Main materials for "Do It Yourself ELISA Kit"
NCBI/Uniprot data below describe general gene information for COL4. It may not necessarily be applicable to this product.
NCBI Accession #
AAD13909.1
[Other Products]
UniProt Secondary Accession #
Q16006; Q16126; Q6LD84; Q7Z700; Q9NUB7[Other Products]
UniProt Related Accession #
P29400[Other Products]
Molecular Weight
161,632 Da
NCBI Official Full Name
collagen type IV alpha 5 chain, partial
NCBI Official Synonym Full Names
collagen type IV alpha 5 chain
NCBI Official Symbol
COL4A5 [Similar Products]
NCBI Official Synonym Symbols
ATS; ASLN; CA54
[Similar Products]
NCBI Protein Information
collagen alpha-5(IV) chain
UniProt Protein Name
Collagen alpha-5(IV) chain
Protein Family
Zinc finger protein CONSTANS
UniProt Gene Name
COL4A5 [Similar Products]
NCBI Summary for COL4
This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]
UniProt Comments for COL4
COL4A5: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X- linked (APSX). APSX is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: Xq22.3
Cellular Component: endoplasmic reticulum lumen; extracellular region
Biological Process: collagen catabolic process; extracellular matrix organization and biogenesis
Disease: Alport Syndrome, X-linked
Research Articles on COL4
1. A) of the COL4A5 gene. Subsequent cDNA analysis revealed that c.547- 3C>A led to skipping of exon 10, which resulted in an in-frame deletion of 21 amino acids from the a5 chain of type IV collagen. This is the first report of the novel c.547-3C>A splicing mutation in the collagen domain of COL4A5 gene.">Sanger sequencing of these regions identified a novel splicesite mutation in intron 9 (c.547-3C>A) of the COL4A5 gene. Subsequent cDNA analysis revealed that c.547- 3C>A led to skipping of exon 10, which resulted in an in-frame deletion of 21 amino acids from the a5 chain of type IV collagen. This is the first report of the novel c.547-3C>A splicing mutation in the collagen domain of COL4A5 gene.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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