CaM Kinase II, alpha, Monoclonal Antibody

Anti -CaM Kinase II, alpha, phosphorylated (Thr286) (Calmodulin-Dependent Protein Kinase II)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 5; NC_000005.9 (149599054..149669403, complement). Location: 5q32

Monoclonal

IgG1

3i29

Mouse

Recognizes phospho-CaM Kinase II a subunit, Mr 50kD. Additional proteins observed at 125kD and 135kD.

Affinity Purified
Purified by Protein G affinity chromatography.

Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-CAMK2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Antibodies; Abs to Calmodulin

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in ELISA and Immunoblot.
Dilution: Immunoblot: 0.5-2.0ug/ml

54,088 Da[Similar Products]

calcium/calmodulin-dependent protein kinase II alpha

calcium/calmodulin-dependent protein kinase type II subunit alpha; CaMKIINalpha; OTTHUMP00000165787; OTTHUMP00000165788; CaMK-II alpha subunit; caMK-II subunit alpha; CaM-kinase II alpha chain; CaM kinase II alpha subunit; caM kinase II subunit alpha; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha

Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, isoform CRA_a

Q7LDD5_HUMAN

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.17; Kinase, protein; CAMK group; CAMK2 family

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: nucleoplasm; presynaptic membrane; mitochondrion; plasma membrane; cell junction; nucleus; cytosol

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; glutamate receptor binding; protein binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; kinase activity; ATP binding

Biological Process: synaptic transmission; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; calcium ion transport; cytokine and chemokine mediated signaling pathway; regulation of neurotransmitter secretion; positive regulation of calcium ion transport; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle; activation of NF-kappaB transcription factor; regulation of neuronal synaptic plasticity

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