Involucrin, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of INV elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS723946 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Involucrin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing INV. The ELISA analytical biochemical technique of the MBS723946 kit is based on INV antibody-INV antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect INV antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, INV. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This IL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: IL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL antibody and an IL-HRP conjugate. The assay sample and buffer are incubated together with IL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL concentration since IL from samples and IL-HRP conjugate compete for the anti-IL antibody . Since the number of sites is limited, as more sites are occupied by IL from the sample, fewer sites are left to bind IL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL concentration in each sample is interpolated from this standard curve.

Signal Transduction

68,479 Da

involucrin

involucrin

Involucrin

INVO_HUMAN

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]

IVL: Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Belongs to the involucrin family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; cornified envelope

Molecular Function: protein binding, bridging; structural molecule activity

Biological Process: keratinocyte differentiation; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; keratinization; peptide cross-linking; response to UV-B

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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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