Product Name
KYNU, Polyclonal Antibody
Popular Item
Full Product Name
KYNU Antibody
Product Synonym Names
Kynureninase; L-kynurenine hydrolase; KYNU
Product Gene Name
anti-KYNU antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q16719
Purity/Purification
Antigen Affinity Purified
Immunogen
Recombinant human Kynureninase protein (1-307AA)
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-KYNU antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-KYNU antibody
Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity.
Applications Tested/Suitable for anti-KYNU antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Western Blot (WB) of anti-KYNU antibody
Western blot
All lanes: KYNUantibody at 3.26ug/ml
Lane 1 : A549 whole cell lysate
Lane 2 : HepG-2 whole cell lysate
Lane 3 : Thp-1 whole cell lysate
Lane 4 : Hela whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 53,35 kDa
Observed band size: 52 kDa
Immunohistochemistry (IHC) of anti-KYNU antibody
Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7045510 at dilution 1:100
Immunoprecipitation (IP) of anti-KYNU antibody
Immunoprecipitating KYNU in HeLa whole cell lysate
Lane 1: Rabbit monoclonal IgG(1ug) instead of MBS7045510 in HeLa whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: MBS7045510(4ug)+ HeLa whole cell lysate(500ug)
Lane 3: HeLa whole cell lysate (20ug)
NCBI/Uniprot data below describe general gene information for KYNU. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001028170.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001032998.1
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UniProt Primary Accession #
Q16719
[Other Products]
UniProt Secondary Accession #
Q6I9T2; Q9BVW3; B2RCZ5; D3DP79[Other Products]
UniProt Related Accession #
Q16719[Other Products]
Molecular Weight
34,635 Da
NCBI Official Full Name
kynureninase isoform b
NCBI Official Synonym Full Names
kynureninase
NCBI Official Symbol
KYNU [Similar Products]
NCBI Official Synonym Symbols
KYNUU
[Similar Products]
NCBI Protein Information
kynureninase
UniProt Protein Name
Kynureninase
UniProt Synonym Protein Names
L-kynurenine hydrolase
Protein Family
Kynureninase
UniProt Gene Name
KYNU [Similar Products]
UniProt Entry Name
KYNU_HUMAN
NCBI Summary for KYNU
Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]
UniProt Comments for KYNU
KYNU: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity. Xanthurenic aciduria manifesting as massive urinary excretion of large amounts of kynurenine, 3-hydroxykynurenine and xanthurenic acid has been observed in an individual carrying a homozygous missense change in KYNU (PubMed:17334708). The urinary pattern in the patient suggests kynureninase deficiency and a block in the conversion of kynurenine and 3-hydroxykynurenine to anthranilate and 3-hydroxyanthranilate, respectively. Belongs to the kynureninase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; Mitochondrial; Amino Acid Metabolism - tryptophan; EC 3.7.1.3
Chromosomal Location of Human Ortholog: 2q22.2
Cellular Component: cytoplasm; cytosol; mitochondrion; nucleoplasm
Molecular Function: kynureninase activity; protein homodimerization activity
Biological Process: anthranilate metabolic process; quinolinate biosynthetic process; tryptophan catabolic process
Disease: Hydroxykynureninuria
Research Articles on KYNU
1. A rare variant at the KYNU gene is associated with essential hypertension in the Han Chinese population.
Precautions
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Disclaimer
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