Kynureninase, ELISA Kit

Kynureninase; L-kynurenine hydrolase; KYNU; 3.7.1.3

For Research Use Only. Not for use in diagnostic procedures.

For long term storage, please store the entire kit at -20 degree C.

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Small volumes of KYNU elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2884314 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Kynureninase, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KYNU. The ELISA analytical biochemical technique of the MBS2884314 kit is based on KYNU antibody-KYNU antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KYNU antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KYNU. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

34,635 Da

kynureninase

kynureninase

Kynureninase

KYNU_HUMAN

Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]

KYNU: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity. Xanthurenic aciduria manifesting as massive urinary excretion of large amounts of kynurenine, 3-hydroxykynurenine and xanthurenic acid has been observed in an individual carrying a homozygous missense change in KYNU (PubMed:17334708). The urinary pattern in the patient suggests kynureninase deficiency and a block in the conversion of kynurenine and 3-hydroxykynurenine to anthranilate and 3-hydroxyanthranilate, respectively. Belongs to the kynureninase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.7.1.3; Amino Acid Metabolism - tryptophan; Hydrolase; Mitochondrial

Chromosomal Location of Human Ortholog: 2q22.2

Cellular Component: cytoplasm; cytosol; mitochondrion; nucleoplasm

Molecular Function: kynureninase activity; protein homodimerization activity; pyridoxal phosphate binding

Biological Process: anthranilate metabolic process; quinolinate biosynthetic process; tryptophan catabolic process; tryptophan catabolic process to acetyl-CoA; tryptophan catabolic process to kynurenine

Disease: Hydroxykynureninuria

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